TY - JOUR
T1 - A somatic reproductive organ enhancer complex activates expression in both the developing and the mature Drosophila reproductive tract
AU - Keplinger, Brian L.
AU - Rabetoy, Anna L.
AU - Cavener, Douglas R.
N1 - Funding Information:
We thank G. Mosig and D. Olsen for critically reading the manuscript. This work was funded by a grant from the National Institutes of Health (GM34170) to D.C. and a predoctoral viruses, nucleic acids and cancer training fellowship from the National Institutes of Health to B.K. The Molecular Dynamics PhosphorImager was funded by a grant from the National Science Foundation (BIR-9419667).
PY - 1996/11/25
Y1 - 1996/11/25
N2 - The Glucose dehydrogenase (Gld) gene is highly expressed in the mature Drosophila reproductive tract. Unlike several other Drosophila genes which function in reproductive physiological processes, Gld is also expressed extensively in the developing reproductive tract during metamorphosis. Proximal promoter elements drive Gld expression in a variety of tissues throughout development, but not in the reproductive tract. Herein, we have identified a somatic reproductive organ enhancer complex (SREC) containing multiple redundant enhancer modules in Gld intron I (+639 to +3906 nt). The SREC, in combination with the Gld promoter, activates βgalactosidase reporter gene expression in both the developing and the mature reproductive tract. The SREC activates a heterologous hsp70 promoter in the ejaculatory duct, but not in other reproductive tract tissues, suggesting that the SREC acts synergistically with Gld promoter proximal elements. Through deletion analysis we have delimited a 361-nt region of the SREC that is involved in ejaculatory duct/oviduct-specific expression. The ejaculatory duct/oviduct enhancer retains the ability to activate expression in both the developing and the mature reproductive tract, suggesting that the same basic enhancer elements activate Gld expression during metamorphosis and in adults. A model of the evolution of Gld expression in the ejaculatory duct and oviduct is presented.
AB - The Glucose dehydrogenase (Gld) gene is highly expressed in the mature Drosophila reproductive tract. Unlike several other Drosophila genes which function in reproductive physiological processes, Gld is also expressed extensively in the developing reproductive tract during metamorphosis. Proximal promoter elements drive Gld expression in a variety of tissues throughout development, but not in the reproductive tract. Herein, we have identified a somatic reproductive organ enhancer complex (SREC) containing multiple redundant enhancer modules in Gld intron I (+639 to +3906 nt). The SREC, in combination with the Gld promoter, activates βgalactosidase reporter gene expression in both the developing and the mature reproductive tract. The SREC activates a heterologous hsp70 promoter in the ejaculatory duct, but not in other reproductive tract tissues, suggesting that the SREC acts synergistically with Gld promoter proximal elements. Through deletion analysis we have delimited a 361-nt region of the SREC that is involved in ejaculatory duct/oviduct-specific expression. The ejaculatory duct/oviduct enhancer retains the ability to activate expression in both the developing and the mature reproductive tract, suggesting that the same basic enhancer elements activate Gld expression during metamorphosis and in adults. A model of the evolution of Gld expression in the ejaculatory duct and oviduct is presented.
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U2 - 10.1006/dbio.1996.0303
DO - 10.1006/dbio.1996.0303
M3 - Article
C2 - 8948593
AN - SCOPUS:0030601993
SN - 0012-1606
VL - 180
SP - 311
EP - 323
JO - Developmental biology
JF - Developmental biology
IS - 1
ER -