TY - JOUR
T1 - A straightforward NMR-spectroscopy-based method for rapid library screening
AU - Siriwardena, Aloysius H.
AU - Tian, Fang
AU - Noble, Schroder
AU - Prestegard, James H.
PY - 2002/9/16
Y1 - 2002/9/16
N2 - An exchange is better than a rest: An NMR screening method is described which facilitates the screening of compound mixtures for components that bind protein drug targets. The approach allows detection of active molecules thanks to a "probe" molecule, which competes with a library of potential ligands for binding sites on a targeted protein. The whole spectrum of active ligands, including those displaying either very fast or very slow exchange behavior, are equally susceptible to detection by this method.
AB - An exchange is better than a rest: An NMR screening method is described which facilitates the screening of compound mixtures for components that bind protein drug targets. The approach allows detection of active molecules thanks to a "probe" molecule, which competes with a library of potential ligands for binding sites on a targeted protein. The whole spectrum of active ligands, including those displaying either very fast or very slow exchange behavior, are equally susceptible to detection by this method.
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U2 - 10.1002/1521-3773(20020916)41:18<3454::AID-ANIE3454>3.0.CO;2-L
DO - 10.1002/1521-3773(20020916)41:18<3454::AID-ANIE3454>3.0.CO;2-L
M3 - Article
C2 - 12298062
AN - SCOPUS:0037119713
SN - 1433-7851
VL - 41
SP - 3454
EP - 3457
JO - Angewandte Chemie - International Edition
JF - Angewandte Chemie - International Edition
IS - 18
ER -