A study on the effect of genotypes, plant growth regulators and sugars in promoting plant regeneration via organogenesis from soybean cotyledonary nodal callus

R. V. Sairam; G. Franklin; R. Hassel; B. Smith; K. Meeker; N. Kashikar; M. Parani; D. Al Abed; S. Ismail; K. Berry; S. L. Goldman

doi:10.1023/A:1024649122748

A study on the effect of genotypes, plant growth regulators and sugars in promoting plant regeneration via organogenesis from soybean cotyledonary nodal callus

R. V. Sairam, G. Franklin, R. Hassel, B. Smith, K. Meeker, N. Kashikar, M. Parani, D. Al Abed, S. Ismail, K. Berry, S. L. Goldman

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42 Scopus citations

Abstract

We have developed an efficient protocol for callus induction and plant regeneration in three elite soybean cultivars (Williams 82, Loda and Newton). The technique is most novel in that the shoot buds developed from the nodal callus. Callus induction and subsequent shoot bud differentiation were achieved from the proximal end of cotyledonary explants on modified Murashige and Skoog (MS) media containing 2.26 μM 2,4-dichlorophenoxy-acetic acid (2,4-D) and 8.8 μM benzyladenine (BAP), respectively. Varying the carbon source optimized the regeneration system further. Among the various carbon sources tested, sorbitol was found to be the best for callus induction and maltose for plant regeneration.

Original language	English (US)
Pages (from-to)	79-85
Number of pages	7
Journal	Plant Cell, Tissue and Organ Culture
Volume	75
Issue number	1
DOIs	https://doi.org/10.1023/A:1024649122748
State	Published - Oct 1 2003

All Science Journal Classification (ASJC) codes

Horticulture

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Sairam, R. V., Franklin, G., Hassel, R., Smith, B., Meeker, K., Kashikar, N., Parani, M., Abed, D. A., Ismail, S., Berry, K., & Goldman, S. L. (2003). A study on the effect of genotypes, plant growth regulators and sugars in promoting plant regeneration via organogenesis from soybean cotyledonary nodal callus. Plant Cell, Tissue and Organ Culture, 75(1), 79-85. https://doi.org/10.1023/A:1024649122748

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title = "A study on the effect of genotypes, plant growth regulators and sugars in promoting plant regeneration via organogenesis from soybean cotyledonary nodal callus",

abstract = "We have developed an efficient protocol for callus induction and plant regeneration in three elite soybean cultivars (Williams 82, Loda and Newton). The technique is most novel in that the shoot buds developed from the nodal callus. Callus induction and subsequent shoot bud differentiation were achieved from the proximal end of cotyledonary explants on modified Murashige and Skoog (MS) media containing 2.26 μM 2,4-dichlorophenoxy-acetic acid (2,4-D) and 8.8 μM benzyladenine (BAP), respectively. Varying the carbon source optimized the regeneration system further. Among the various carbon sources tested, sorbitol was found to be the best for callus induction and maltose for plant regeneration.",

author = "Sairam, {R. V.} and G. Franklin and R. Hassel and B. Smith and K. Meeker and N. Kashikar and M. Parani and Abed, {D. Al} and S. Ismail and K. Berry and Goldman, {S. L.}",

note = "Funding Information: The authors gratefully acknowledge the support from USDA-ARS grant 3607-2100-008-01S.",

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Sairam, RV, Franklin, G, Hassel, R, Smith, B, Meeker, K, Kashikar, N, Parani, M, Abed, DA, Ismail, S, Berry, K & Goldman, SL 2003, 'A study on the effect of genotypes, plant growth regulators and sugars in promoting plant regeneration via organogenesis from soybean cotyledonary nodal callus', Plant Cell, Tissue and Organ Culture, vol. 75, no. 1, pp. 79-85. https://doi.org/10.1023/A:1024649122748

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T1 - A study on the effect of genotypes, plant growth regulators and sugars in promoting plant regeneration via organogenesis from soybean cotyledonary nodal callus

AU - Sairam, R. V.

AU - Franklin, G.

AU - Hassel, R.

AU - Smith, B.

AU - Meeker, K.

AU - Kashikar, N.

AU - Parani, M.

AU - Abed, D. Al

AU - Ismail, S.

AU - Berry, K.

AU - Goldman, S. L.

N1 - Funding Information: The authors gratefully acknowledge the support from USDA-ARS grant 3607-2100-008-01S.

PY - 2003/10/1

Y1 - 2003/10/1

N2 - We have developed an efficient protocol for callus induction and plant regeneration in three elite soybean cultivars (Williams 82, Loda and Newton). The technique is most novel in that the shoot buds developed from the nodal callus. Callus induction and subsequent shoot bud differentiation were achieved from the proximal end of cotyledonary explants on modified Murashige and Skoog (MS) media containing 2.26 μM 2,4-dichlorophenoxy-acetic acid (2,4-D) and 8.8 μM benzyladenine (BAP), respectively. Varying the carbon source optimized the regeneration system further. Among the various carbon sources tested, sorbitol was found to be the best for callus induction and maltose for plant regeneration.

AB - We have developed an efficient protocol for callus induction and plant regeneration in three elite soybean cultivars (Williams 82, Loda and Newton). The technique is most novel in that the shoot buds developed from the nodal callus. Callus induction and subsequent shoot bud differentiation were achieved from the proximal end of cotyledonary explants on modified Murashige and Skoog (MS) media containing 2.26 μM 2,4-dichlorophenoxy-acetic acid (2,4-D) and 8.8 μM benzyladenine (BAP), respectively. Varying the carbon source optimized the regeneration system further. Among the various carbon sources tested, sorbitol was found to be the best for callus induction and maltose for plant regeneration.

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A study on the effect of genotypes, plant growth regulators and sugars in promoting plant regeneration via organogenesis from soybean cotyledonary nodal callus

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