ABCG2 transporter reduces protein aggregation in cigarette smoke condensate-exposed A549 lung cancer cells

Emmanuella O. Ajenu, Ashley M. Seideneck, Esh Pandellapalli, Emily M. Shinsky, Casey L. Humphries, Nicholas L. Aparicio, Mahak Sharma, James H. Marden, Maria M. Krasilnikova

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Cigarette smoke-induced protein aggregation damages the lung cells in emphysema and COPD; however, lung cancer cells continue to thrive, evolving to persist in the toxic environment. Here, we showed that upon the cigarette smoke condensate exposure, A549 lung cancer cells exhibit better survival and reduced level of protein aggregation when compared to non-cancerous Beas-2B and H-6053 cells. Our data suggests that upregulation of efflux pumps in cancer cells assists in reducing smoke toxicity. Specifically, we demonstrated that inhibition of the ABCG2 transporter in A549 by febuxostat or its downregulation by shRNA-mediated RNA interference resulted in a significant increase in protein aggregation due to smoke exposure.

Original languageEnglish (US)
Article numbere0297661
JournalPloS one
Issue number3 March
StatePublished - Mar 2024

All Science Journal Classification (ASJC) codes

  • General

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