TY - JOUR
T1 - Activated natural killer cells adhere to cultured hippocampal neurons and affect the dendritic morphology
AU - Hasegawa, Sanae
AU - Yamaguchi, Masahiro
AU - Nagao, Hiroshi
AU - Yoshihara, Yoshihiro
AU - Mori, Kensaku
N1 - Funding Information:
This work was supported by Grants-in-Aid for Creative Scientific Research and Grant-in-Aid for Exploratory Research from JSPS and Grant-in-Aid for JSPS Fellows. We thank H. Matsuno, K. Kubota, T. Nakata, F. Imamura, K. Inaki and colleagues in the department of physiology for advices and valuable discussions.
PY - 2004/6
Y1 - 2004/6
N2 - To examine the manner of interactions between immune cells and central nervous system (CNS) neurons, mouse hippocampal neurons were co-cultured with lymphokine (IL-2)-activated killer (LAK) cells. Immunocytochemical and time-lapse observations indicated that LAK cells migrated along neuronal processes and made adhesive contacts with them. In addition to the direct physical effects, LAK cells released glutamate, induced the formation of beads-like structure in the dendrites of about 14% of hippocampal neurons and caused the reduction of dendritic protrusions. These results suggest that infiltrating immune cells can form direct adhesive connections with CNS neurons and affect their dendritic morphology.
AB - To examine the manner of interactions between immune cells and central nervous system (CNS) neurons, mouse hippocampal neurons were co-cultured with lymphokine (IL-2)-activated killer (LAK) cells. Immunocytochemical and time-lapse observations indicated that LAK cells migrated along neuronal processes and made adhesive contacts with them. In addition to the direct physical effects, LAK cells released glutamate, induced the formation of beads-like structure in the dendrites of about 14% of hippocampal neurons and caused the reduction of dendritic protrusions. These results suggest that infiltrating immune cells can form direct adhesive connections with CNS neurons and affect their dendritic morphology.
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U2 - 10.1016/j.jneuroim.2004.03.002
DO - 10.1016/j.jneuroim.2004.03.002
M3 - Article
C2 - 15145611
AN - SCOPUS:2442474163
SN - 0165-5728
VL - 151
SP - 126
EP - 136
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
IS - 1-2
ER -