Activated natural killer cells adhere to cultured hippocampal neurons and affect the dendritic morphology

Sanae Hasegawa; Masahiro Yamaguchi; Hiroshi Nagao; Yoshihiro Yoshihara; Kensaku Mori

doi:10.1016/j.jneuroim.2004.03.002

Activated natural killer cells adhere to cultured hippocampal neurons and affect the dendritic morphology

Sanae Hasegawa, Masahiro Yamaguchi, Hiroshi Nagao, Yoshihiro Yoshihara, Kensaku Mori

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

To examine the manner of interactions between immune cells and central nervous system (CNS) neurons, mouse hippocampal neurons were co-cultured with lymphokine (IL-2)-activated killer (LAK) cells. Immunocytochemical and time-lapse observations indicated that LAK cells migrated along neuronal processes and made adhesive contacts with them. In addition to the direct physical effects, LAK cells released glutamate, induced the formation of beads-like structure in the dendrites of about 14% of hippocampal neurons and caused the reduction of dendritic protrusions. These results suggest that infiltrating immune cells can form direct adhesive connections with CNS neurons and affect their dendritic morphology.

Original language	English (US)
Pages (from-to)	126-136
Number of pages	11
Journal	Journal of Neuroimmunology
Volume	151
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jneuroim.2004.03.002
State	Published - Jun 2004

All Science Journal Classification (ASJC) codes

Immunology and Allergy
Immunology
Neurology
Clinical Neurology

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10.1016/j.jneuroim.2004.03.002

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@article{a56fcaf135014232be7a426b90e7cfc9,

title = "Activated natural killer cells adhere to cultured hippocampal neurons and affect the dendritic morphology",

abstract = "To examine the manner of interactions between immune cells and central nervous system (CNS) neurons, mouse hippocampal neurons were co-cultured with lymphokine (IL-2)-activated killer (LAK) cells. Immunocytochemical and time-lapse observations indicated that LAK cells migrated along neuronal processes and made adhesive contacts with them. In addition to the direct physical effects, LAK cells released glutamate, induced the formation of beads-like structure in the dendrites of about 14% of hippocampal neurons and caused the reduction of dendritic protrusions. These results suggest that infiltrating immune cells can form direct adhesive connections with CNS neurons and affect their dendritic morphology.",

author = "Sanae Hasegawa and Masahiro Yamaguchi and Hiroshi Nagao and Yoshihiro Yoshihara and Kensaku Mori",

note = "Funding Information: This work was supported by Grants-in-Aid for Creative Scientific Research and Grant-in-Aid for Exploratory Research from JSPS and Grant-in-Aid for JSPS Fellows. We thank H. Matsuno, K. Kubota, T. Nakata, F. Imamura, K. Inaki and colleagues in the department of physiology for advices and valuable discussions.",

year = "2004",

month = jun,

doi = "10.1016/j.jneuroim.2004.03.002",

language = "English (US)",

volume = "151",

pages = "126--136",

journal = "Journal of Neuroimmunology",

issn = "0165-5728",

publisher = "Elsevier B.V.",

number = "1-2",

}

TY - JOUR

T1 - Activated natural killer cells adhere to cultured hippocampal neurons and affect the dendritic morphology

AU - Hasegawa, Sanae

AU - Yamaguchi, Masahiro

AU - Nagao, Hiroshi

AU - Yoshihara, Yoshihiro

AU - Mori, Kensaku

N1 - Funding Information: This work was supported by Grants-in-Aid for Creative Scientific Research and Grant-in-Aid for Exploratory Research from JSPS and Grant-in-Aid for JSPS Fellows. We thank H. Matsuno, K. Kubota, T. Nakata, F. Imamura, K. Inaki and colleagues in the department of physiology for advices and valuable discussions.

PY - 2004/6

Y1 - 2004/6

N2 - To examine the manner of interactions between immune cells and central nervous system (CNS) neurons, mouse hippocampal neurons were co-cultured with lymphokine (IL-2)-activated killer (LAK) cells. Immunocytochemical and time-lapse observations indicated that LAK cells migrated along neuronal processes and made adhesive contacts with them. In addition to the direct physical effects, LAK cells released glutamate, induced the formation of beads-like structure in the dendrites of about 14% of hippocampal neurons and caused the reduction of dendritic protrusions. These results suggest that infiltrating immune cells can form direct adhesive connections with CNS neurons and affect their dendritic morphology.

AB - To examine the manner of interactions between immune cells and central nervous system (CNS) neurons, mouse hippocampal neurons were co-cultured with lymphokine (IL-2)-activated killer (LAK) cells. Immunocytochemical and time-lapse observations indicated that LAK cells migrated along neuronal processes and made adhesive contacts with them. In addition to the direct physical effects, LAK cells released glutamate, induced the formation of beads-like structure in the dendrites of about 14% of hippocampal neurons and caused the reduction of dendritic protrusions. These results suggest that infiltrating immune cells can form direct adhesive connections with CNS neurons and affect their dendritic morphology.

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SN - 0165-5728

VL - 151

SP - 126

EP - 136

JO - Journal of Neuroimmunology

JF - Journal of Neuroimmunology

IS - 1-2

ER -

Activated natural killer cells adhere to cultured hippocampal neurons and affect the dendritic morphology

Abstract

All Science Journal Classification (ASJC) codes

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