Activation of src family kinase lck following CD28 crosslinking in the jurkat leukemic cell line

Avery August, Bo Dupont

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59 Scopus citations


T lymphocytes require a signal via their antigen specific receptors (the T cell receptors) and an antigen independent costimulatory signal. Signals through CD28 can costimulate T cells in the presence of limiting amounts of T cell receptor signal, or in the presence of PMA, providing ths second signal. CD28 signaling is known to involve the activation of protein tyrosine kinases. Using the Jurkat leukemic cell line as a model we have tested CD28 crosslinking for its effects on the protein tyrosine kinases p56lck We report that following crosslinking of CD28, p56lck kinase activity is increased. Crosslinking CD28 causes a shift in the relative mobility of p56lck from 56 to 60 kD similar to that seen after crosslinking of CD2 and CD4, cell surface receptors known to be associate with and activate p56lck. Finally, lck could be found in anti-CD28 immunoprecipitates in exponentially growing Jurkat and in 'activated' CD28 (i.e., cross linked), but not in CD28 in resting Jurkat cells. These findings suggest an important role for p56lck in CD28 signal transduction.

Original languageEnglish (US)
Pages (from-to)1466-1473
Number of pages8
JournalBiochemical and Biophysical Research Communications
Issue number3
StatePublished - Mar 30 1994

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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