TY - JOUR
T1 - Active cell-matrix coupling regulates cellular force landscapes of cohesive epithelial monolayers
AU - Zhao, Tiankai
AU - Zhang, Yao
AU - Wei, Qiong
AU - Shi, Xuechen
AU - Zhao, Peng
AU - Chen, Long Qing
AU - Zhang, Sulin
N1 - Funding Information:
S.L.Z. acknowledges support by the National Science Foundation (NSF) under Grants CMMI-0754463 and CBET-1067523 and by the National Institute of Health (NIH) under the grant R21HL122902.
Publisher Copyright:
© 2018 The Author(s).
PY - 2018/12/1
Y1 - 2018/12/1
N2 - Epithelial cells can assemble into cohesive monolayers with rich morphologies on substrates due to competition between elastic, edge, and interfacial effects. Here we present a molecularly based thermodynamic model, integrating monolayer and substrate elasticity, and force-mediated focal adhesion formation, to elucidate the active biochemical regulation over the cellular force landscapes in cohesive epithelial monolayers, corroborated by microscopy and immunofluorescence studies. The predicted extracellular traction and intercellular tension are both monolayer size and substrate stiffness dependent, suggestive of cross-talks between intercellular and extracellular activities. Our model sets a firm ground toward a versatile computational framework to uncover the molecular origins of morphogenesis and disease in multicellular epithelia.
AB - Epithelial cells can assemble into cohesive monolayers with rich morphologies on substrates due to competition between elastic, edge, and interfacial effects. Here we present a molecularly based thermodynamic model, integrating monolayer and substrate elasticity, and force-mediated focal adhesion formation, to elucidate the active biochemical regulation over the cellular force landscapes in cohesive epithelial monolayers, corroborated by microscopy and immunofluorescence studies. The predicted extracellular traction and intercellular tension are both monolayer size and substrate stiffness dependent, suggestive of cross-talks between intercellular and extracellular activities. Our model sets a firm ground toward a versatile computational framework to uncover the molecular origins of morphogenesis and disease in multicellular epithelia.
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U2 - 10.1038/s41524-018-0069-8
DO - 10.1038/s41524-018-0069-8
M3 - Article
AN - SCOPUS:85044179691
SN - 2057-3960
VL - 4
JO - npj Computational Materials
JF - npj Computational Materials
IS - 1
M1 - 10
ER -