Administration of recombinant human insulin-like growth factor I to pigs: Determination of circulating half-lives and chomatographic profiles

P. E. Walton; R. Gopinath; B. D. Burleigh; T. D. Etherton

doi:10.1159/000181103

Administration of recombinant human insulin-like growth factor I to pigs: Determination of circulating half-lives and chomatographic profiles

P. E. Walton, R. Gopinath, B. D. Burleigh, T. D. Etherton

Animal Science

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14 Scopus citations

Abstract

Recombinant human insulin-like growth factor I (IGF-I) was injected daily (4 or 8 mg/pig) as an intra-arterial bolus into pigs for 3 consecutive days and the serum IGF-I concentration was measured to determine disappearance profiles. IGF-I partitioned to a fast component (half-life, t( 1/2 ) = 5.7 min) and a slow component (t( 1/2 ) = 253 min) in pig serum. Chromatography of serum revealed that the fast component comprised unbound IGF-I, whereas the slow component comprised IGF-I bound to 40- and 150-kD serum IGF-binding proteins. In addition, administration of exogenous IGF-I caused significant hypoglycemia.

Original language	English (US)
Pages (from-to)	138-142
Number of pages	5
Journal	Hormone Research
Volume	31
Issue number	3
DOIs	https://doi.org/10.1159/000181103
State	Published - 1989

All Science Journal Classification (ASJC) codes

Endocrinology, Diabetes and Metabolism
Endocrinology

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10.1159/000181103

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abstract = "Recombinant human insulin-like growth factor I (IGF-I) was injected daily (4 or 8 mg/pig) as an intra-arterial bolus into pigs for 3 consecutive days and the serum IGF-I concentration was measured to determine disappearance profiles. IGF-I partitioned to a fast component (half-life, t( 1/2 ) = 5.7 min) and a slow component (t( 1/2 ) = 253 min) in pig serum. Chromatography of serum revealed that the fast component comprised unbound IGF-I, whereas the slow component comprised IGF-I bound to 40- and 150-kD serum IGF-binding proteins. In addition, administration of exogenous IGF-I caused significant hypoglycemia.",

author = "Walton, {P. E.} and R. Gopinath and Burleigh, {B. D.} and Etherton, {T. D.}",

year = "1989",

doi = "10.1159/000181103",

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AU - Gopinath, R.

AU - Burleigh, B. D.

AU - Etherton, T. D.

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AB - Recombinant human insulin-like growth factor I (IGF-I) was injected daily (4 or 8 mg/pig) as an intra-arterial bolus into pigs for 3 consecutive days and the serum IGF-I concentration was measured to determine disappearance profiles. IGF-I partitioned to a fast component (half-life, t( 1/2 ) = 5.7 min) and a slow component (t( 1/2 ) = 253 min) in pig serum. Chromatography of serum revealed that the fast component comprised unbound IGF-I, whereas the slow component comprised IGF-I bound to 40- and 150-kD serum IGF-binding proteins. In addition, administration of exogenous IGF-I caused significant hypoglycemia.

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Administration of recombinant human insulin-like growth factor I to pigs: Determination of circulating half-lives and chomatographic profiles

Abstract

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