TY - JOUR
T1 - Advances in wound-healing assays for probing collective cell migration
AU - Riahi, Reza
AU - Yang, Yongliang
AU - Zhang, Donna D.
AU - Wong, Pak Kin
N1 - Funding Information:
The author(s) disclosed receipt of the following financial support for the research and/or authorship of this article: R.R. is partially supported by the University of Arizona TRIF Imaging fellowship. This work is supported by the National Institutes of Health Director’s New Innovator Award (1DP2OD007161-01), the National Science Foundation (0855890), and the James S. McDonnell Foundation.
PY - 2012/2
Y1 - 2012/2
N2 - Collective cell migration plays essential roles in a wide spectrum of biological processes, such as embryogenesis, tissue regeneration, and cancer metastasis. Numerous wound-healing assays based on mechanical, chemical, optical, and electrical approaches have been developed to create model "wounds" in cell monolayers to study the collective cell migration processes. These approaches can result in different microenvironments for cells to migrate and possess diverse assay characteristics in terms of simplicity, throughput, reproducibility, and multiplexability. In this review, we provide an overview of advances in wound-healing assays and discuss their advantages and limitations in studying collective cell migration.
AB - Collective cell migration plays essential roles in a wide spectrum of biological processes, such as embryogenesis, tissue regeneration, and cancer metastasis. Numerous wound-healing assays based on mechanical, chemical, optical, and electrical approaches have been developed to create model "wounds" in cell monolayers to study the collective cell migration processes. These approaches can result in different microenvironments for cells to migrate and possess diverse assay characteristics in terms of simplicity, throughput, reproducibility, and multiplexability. In this review, we provide an overview of advances in wound-healing assays and discuss their advantages and limitations in studying collective cell migration.
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U2 - 10.1177/2211068211426550
DO - 10.1177/2211068211426550
M3 - Review article
C2 - 22357609
AN - SCOPUS:84864741030
SN - 2211-0682
VL - 17
SP - 59
EP - 65
JO - Journal of Laboratory Automation
JF - Journal of Laboratory Automation
IS - 1
ER -