Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice

Liza Anastasia Dicecco, Samantha Berry, G. M. Jonaid, Maria J. Solares, Liam Kaylor, Jennifer L. Gray, Carol Bator, William J. Dearnaley, Michael Spilman, Madeline J. Dressel-Dukes, Kathryn Grandfield, Sarah M. McDonald Esstman, Deborah F. Kelly

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Interest in liquid-electron microscopy (liquid-EM) has skyrocketed in recent years as scientists can now observe real-time processes at the nanoscale. It is extremely desirable to pair high-resolution cryo-EM information with dynamic observations as many events occur at rapid timescales-in the millisecond range or faster. Improved knowledge of flexible structures can also assist in the design of novel reagents to combat emerging pathogens, such as SARS-CoV-2. More importantly, viewing biological materials in a fluid environment provides a unique glimpse of their performance in the human body. Presented here are newly developed methods to investigate the nanoscale properties of virus assemblies in liquid and vitreous ice. To accomplish this goal, well-defined samples were used as model systems. Side-by-side comparisons of sample preparation methods and representative structural information are presented. Sub-nanometer features are shown for structures resolved in the range of ~3.5-Å-10 Å. Other recent results that support this complementary framework include dynamic insights of vaccine candidates and antibody-based therapies imaged in liquid. Overall, these correlative applications advance our ability to visualize molecular dynamics, providing a unique context for their use in human health and disease.

Original languageEnglish (US)
Article numbere63856
JournalJournal of Visualized Experiments
Volume2022
Issue number185
DOIs
StatePublished - Jul 2022

All Science Journal Classification (ASJC) codes

  • General Neuroscience
  • General Chemical Engineering
  • General Biochemistry, Genetics and Molecular Biology
  • General Immunology and Microbiology

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