Adventures in trying to understand enzymic catalysis

Stephen Benkovic, J. Huang, S. Singleton, A. Nixon, M. Warren

Research output: Contribution to journalArticlepeer-review


The complexities and marvels of enzymic catalysis will be illustrated for three cases: first, the importance of conformational flexibility in the mode of action of dihydrofolate reductase analyzed through a combined kinetic and mutagenic examination of function; second, the uniqueness of the nature and locus of active site side chains as revealed by the result of saturation mutagenesis of these amino acids in glycinamide ribonucleotide transformylase; and third, the implications for molecular recruitment in achieving enzyme function through the construction of hybrid genes from genes associated with the purine biosynthetic pathway.

Original languageEnglish (US)
JournalFASEB Journal
Issue number6
StatePublished - Dec 1 1996

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics


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