Affinity electrophoresis for analysis of catalytic module-carbohydrate interactions

Darrell Cockburn, Casper Wilkens, Birte Svensson

Research output: Chapter in Book/Report/Conference proceedingChapter

9 Scopus citations

Abstract

Affinity electrophoresis has long been used to study the interaction between proteins and large soluble ligands. The technique has been found to have great utility for the examination of polysaccharide binding by proteins, particularly carbohydrate binding modules (CBMs). In recent years, carbohydrate surface binding sites of proteins mostly enzymes have also been investigated by this method. Here, we describe a protocol for identifying binding interactions between enzyme catalytic modules and a variety of carbohydrate ligands.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages119-127
Number of pages9
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1588
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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