Affinity Electrophoresis for Analysis of Catalytic Module-Carbohydrate Interactions

Darrell W. Cockburn, Casper Wilkens, Birte Svensson

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Affinity electrophoresis has long been used to study the interaction between proteins and large soluble ligands. The technique has been found to have great utility for the examination of polysaccharide binding by proteins, particularly carbohydrate-binding modules (CBMs). In recent years carbohydrate surface binding sites of proteins, mostly enzymes, have also been investigated by this method. Here we describe a protocol for identifying binding interactions between enzyme catalytic modules and a variety of carbohydrate ligands.

Original languageEnglish (US)
Pages (from-to)91-101
Number of pages11
JournalMethods in molecular biology (Clifton, N.J.)
StatePublished - 2023

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics


Dive into the research topics of 'Affinity Electrophoresis for Analysis of Catalytic Module-Carbohydrate Interactions'. Together they form a unique fingerprint.

Cite this