TY - JOUR
T1 - Alaskan seaweeds lower inflammation in RAW 264.7 macrophages and decrease lipid accumulation in 3T3-L1 adipocytes
AU - Kellogg, Joshua
AU - Esposito, Debora
AU - Grace, Mary H.
AU - Komarnytsky, Slavko
AU - Lila, Mary Ann
N1 - Funding Information:
The authors wish to thank Renae Matheson and the Southeast Alaska Regional Health Consortium (SEARHC) for assistance in collecting and screening the species used in this study. We also would like to acknowledge the assistance of Lorie Solomon-Beale and Rennetta Roberts of the Plants for Human Health Institute for their technical help in the cell culture and in vitro assays. This project is supported with funding from the UNC General Administration and NC State University's Plants for Human Health Institute .
Publisher Copyright:
© 2015 Elsevier Ltd.
PY - 2015/5/1
Y1 - 2015/5/1
N2 - Chronic inflammation is characterized by macrophage accumulation in adipose tissue, which subsequently up-regulates pro-inflammatory cytokines and promotes the dysregulation of lipid metabolism, ultimately leading to insulin resistance. This study was designed to examine the effects of coastal Alaskan seaweeds on the macrophage inflammatory response and lipid metabolism of adipocytes. Two bioactive subfractions from the brown alga Fucus distichus, a monoglycosyldiacylglycerol subfraction and a phlorotannin subfraction, decreased mRNA expression of acute and chronic inflammatory biomarkers. Expression of Toll-like receptors TLR4 and TLR9 were also reduced, suggesting a potential mechanism of anti-inflammatory activity via TLR attenuation. F. distichus fractions decreased lipid accumulation up to 55% and increased free glycerol concentrations by 28-45%. This result was supported by increases in adiponectin and UCP-1 and decreases in leptin mRNA expression. Overall, the Alaskan seaweed F. distichus inhibited proinflammatory responses and improved lipid metabolism, suggesting the potential for seaweed phytochemicals to attenuate inflammatory diseases.
AB - Chronic inflammation is characterized by macrophage accumulation in adipose tissue, which subsequently up-regulates pro-inflammatory cytokines and promotes the dysregulation of lipid metabolism, ultimately leading to insulin resistance. This study was designed to examine the effects of coastal Alaskan seaweeds on the macrophage inflammatory response and lipid metabolism of adipocytes. Two bioactive subfractions from the brown alga Fucus distichus, a monoglycosyldiacylglycerol subfraction and a phlorotannin subfraction, decreased mRNA expression of acute and chronic inflammatory biomarkers. Expression of Toll-like receptors TLR4 and TLR9 were also reduced, suggesting a potential mechanism of anti-inflammatory activity via TLR attenuation. F. distichus fractions decreased lipid accumulation up to 55% and increased free glycerol concentrations by 28-45%. This result was supported by increases in adiponectin and UCP-1 and decreases in leptin mRNA expression. Overall, the Alaskan seaweed F. distichus inhibited proinflammatory responses and improved lipid metabolism, suggesting the potential for seaweed phytochemicals to attenuate inflammatory diseases.
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U2 - 10.1016/j.jff.2015.03.049
DO - 10.1016/j.jff.2015.03.049
M3 - Article
AN - SCOPUS:84929231499
SN - 1756-4646
VL - 15
SP - 396
EP - 407
JO - Journal of Functional Foods
JF - Journal of Functional Foods
ER -