TY - JOUR
T1 - Alpha-subunit positions methionine 180 and glutamate 214 of Pseudomonas stutzeri OX1 toluene-o-xylene monooxygenase influence catalysis
AU - Vardar, Gönül
AU - Wood, Thomas K.
PY - 2005/2
Y1 - 2005/2
N2 - Alpha-subunit position M180 of toluene-o-xylene monooxygenase influences the regiospecific oxidation of aromatics (e.g., from o-cresol, M180H forms 3-methylcatechol, methylhydroquinone, and 4-methylresorcinol, whereas the wild type forms only 3-methylcatechol). Position E214 influences the rate of reaction (e.g., E214G increases p-nitrophenol oxidation 15-fold) by controlling substrate entrance and product efflux as a gate residue.
AB - Alpha-subunit position M180 of toluene-o-xylene monooxygenase influences the regiospecific oxidation of aromatics (e.g., from o-cresol, M180H forms 3-methylcatechol, methylhydroquinone, and 4-methylresorcinol, whereas the wild type forms only 3-methylcatechol). Position E214 influences the rate of reaction (e.g., E214G increases p-nitrophenol oxidation 15-fold) by controlling substrate entrance and product efflux as a gate residue.
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U2 - 10.1128/JB.187.4.1511-1514.2005
DO - 10.1128/JB.187.4.1511-1514.2005
M3 - Article
C2 - 15687216
AN - SCOPUS:14544277660
SN - 0021-9193
VL - 187
SP - 1511
EP - 1514
JO - Journal of bacteriology
JF - Journal of bacteriology
IS - 4
ER -