TY - JOUR
T1 - Alterations in the MA and NC domains modulate phosphoinositide-dependent plasma membrane localization of the Rous sarcoma virus gag protein
AU - Nadaraia-Hoke, Shorena
AU - Bann, Darrin V.
AU - Lochmann, Timothy L.
AU - Gudleski-O'Regan, Nicole
AU - Parent, Leslie J.
PY - 2013/3
Y1 - 2013/3
N2 - Retroviral Gag proteins direct virus particle assembly from the plasma membrane (PM). Phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2] plays a role in PM targeting of several retroviral Gag proteins. Here we report that depletion of intracellular PI(4,5)P2 and phosphatidylinositol-(3,4,5)-triphosphate [PI(3,4,5)P3] levels impaired Rous sarcoma virus (RSV) Gag PM localization. Gag mutants deficient in nuclear trafficking were less sensitive to reduction of intracellular PI(4,5)P2 and PI(3,4,5)P3,suggesting a possible connection between Gag nuclear trafficking and phosphoinositide-dependent PM targeting.
AB - Retroviral Gag proteins direct virus particle assembly from the plasma membrane (PM). Phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2] plays a role in PM targeting of several retroviral Gag proteins. Here we report that depletion of intracellular PI(4,5)P2 and phosphatidylinositol-(3,4,5)-triphosphate [PI(3,4,5)P3] levels impaired Rous sarcoma virus (RSV) Gag PM localization. Gag mutants deficient in nuclear trafficking were less sensitive to reduction of intracellular PI(4,5)P2 and PI(3,4,5)P3,suggesting a possible connection between Gag nuclear trafficking and phosphoinositide-dependent PM targeting.
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U2 - 10.1128/JVI.03059-12
DO - 10.1128/JVI.03059-12
M3 - Article
C2 - 23325682
AN - SCOPUS:84874678574
SN - 0022-538X
VL - 87
SP - 3609
EP - 3615
JO - Journal of virology
JF - Journal of virology
IS - 6
ER -