TY - JOUR
T1 - Amperometric sensing of norepinephrine at picomolar concentrations using screen printed, high surface area mesoporous carbon
AU - Dai, Mingzhi
AU - Haselwood, Brittney
AU - Vogt, Bryan D.
AU - La Belle, Jeffrey T.
N1 - Funding Information:
This work is partially financially supported by the National Science Foundation under grant CBET-074664 and partially supported by Fulton School of Engineering through the Fulton Undergraduate Research initiative at Arizona State University. The authors thank the support of the Center for Solid State Electronics Research and Center for Solid State Science for research facilities used in this work.
PY - 2013/7/25
Y1 - 2013/7/25
N2 - Norepinephrine (NE) is detected amperometrically using the enzyme Phenylethanolamine N-methyl transferase and cofactor S-(5'-Adenosyl)-l-methionine chloride dihydrochloride with disposable screen printed mesoporous carbon electrodes. The role of internal surface area and pore size of the mesoporous carbon is systematically examined using soft-templated, mesoporous silica-carbon powders with highly microporous walls obtained from etching of the silica to produce powders with surface areas ranging from 671-2339m2g-1. As the surface area increases, the sensitivity of the biosensor at very low NE concentrations (0-500pgmL-1) in phosphate buffered saline (PBS) increases just as the current signal increases with respect to the NE concentration of 81-1581μAmLng-1cm-2 for the mesoporous carbons. The best performing electrode provides similar sensitivity in whole rabbit blood in comparison to PBS despite no membrane layer to filter the non-desired reactants; the small (<5nm) pore size and large internal surface area acts to minimize non-specific events that decrease sensitivity.
AB - Norepinephrine (NE) is detected amperometrically using the enzyme Phenylethanolamine N-methyl transferase and cofactor S-(5'-Adenosyl)-l-methionine chloride dihydrochloride with disposable screen printed mesoporous carbon electrodes. The role of internal surface area and pore size of the mesoporous carbon is systematically examined using soft-templated, mesoporous silica-carbon powders with highly microporous walls obtained from etching of the silica to produce powders with surface areas ranging from 671-2339m2g-1. As the surface area increases, the sensitivity of the biosensor at very low NE concentrations (0-500pgmL-1) in phosphate buffered saline (PBS) increases just as the current signal increases with respect to the NE concentration of 81-1581μAmLng-1cm-2 for the mesoporous carbons. The best performing electrode provides similar sensitivity in whole rabbit blood in comparison to PBS despite no membrane layer to filter the non-desired reactants; the small (<5nm) pore size and large internal surface area acts to minimize non-specific events that decrease sensitivity.
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U2 - 10.1016/j.aca.2013.06.019
DO - 10.1016/j.aca.2013.06.019
M3 - Article
C2 - 23845478
AN - SCOPUS:84880036230
SN - 0003-2670
VL - 788
SP - 32
EP - 38
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
ER -