TY - JOUR
T1 - Amphetamine redistributes dopamine from synaptic vesicles to the cytosol and promotes reverse transport
AU - Sulzer, David
AU - Chen, Ta Kung
AU - Lau, Yau Yi
AU - Kristensen, Helle
AU - Rayport, Stephen
AU - Ewing, Andrew
PY - 1995/5
Y1 - 1995/5
N2 - Whether amphetamine acts principally at the plasma membrane or at synaptic vesicles is controversial. We find that d-amphetamine injection into the Planorbis giant dopamine neuron causes robust dopamine release, demonstrating that specific amphetamine uptake is not required. Arguing for action at vesicles, whole-cell capillary electrophoresis of single Planorbis dopamine neurons shows that amphetamine reduces vesicular dopamine, while amphetamine reduces quantal dopamine release from PC12 cells by > 50% per vesicle. Intracellular injection of dopamine into the Planorbis dopamine neuron produces rapid nomifensine-sensitive release, showing that an increased substrate concentration gradient is sufficient to induce release. These experiments indicate that amphetamine acts at the vesicular level where it redistributes dopamine to the cytosol, promoting reverse transport, and dopamine release.
AB - Whether amphetamine acts principally at the plasma membrane or at synaptic vesicles is controversial. We find that d-amphetamine injection into the Planorbis giant dopamine neuron causes robust dopamine release, demonstrating that specific amphetamine uptake is not required. Arguing for action at vesicles, whole-cell capillary electrophoresis of single Planorbis dopamine neurons shows that amphetamine reduces vesicular dopamine, while amphetamine reduces quantal dopamine release from PC12 cells by > 50% per vesicle. Intracellular injection of dopamine into the Planorbis dopamine neuron produces rapid nomifensine-sensitive release, showing that an increased substrate concentration gradient is sufficient to induce release. These experiments indicate that amphetamine acts at the vesicular level where it redistributes dopamine to the cytosol, promoting reverse transport, and dopamine release.
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U2 - 10.1523/jneurosci.15-05-04102.1995
DO - 10.1523/jneurosci.15-05-04102.1995
M3 - Article
C2 - 7751968
AN - SCOPUS:0029032794
SN - 0270-6474
VL - 15
SP - 4102
EP - 4108
JO - Journal of Neuroscience
JF - Journal of Neuroscience
IS - 5 II
ER -