An AFLP linkage map using a hybrid population of Populus tomentosa

The map was constructed using Populus tomentosa × P . alba var. pyramidalis × P . tomentosa hybrid population in which the resistance of Dothrorella gregaria Sacc was segregated. The authors had obtained 662 markers using 19 Pstl/Msel primer pairs and 4 EcoRI/Msel primer pairs, of which 43.2% had 3:1 in segregation, 14.5% appears to be non-parental heteroduplex, and 48.8% was distorted.239 markers had been mapped into 22 linkage groups (each including 4 loci or more), and the integrated map was 4 418 cM in length, with an average locus interval of 18.5 cM. Linkage group LGI was the largest one and covered 524 cM with 25 markers. The densest linkage group was LG13 with an average interval of 6.7 cM in length. There were duplicated loci for 8 markers, and 7 markers that linked to one of markers in a linkage group but couldn't be mapped into that group. In addition, there were 15 minor groups harboring 2 ∼ 3 markers belonged to the parents. Moreover, 13 and 21 groups belonged to paternal and maternal parent, respectively.