An approach to histone nearest neighbours in extended chromatin

Ross C. Hardison; Mary Ellen Eichner; Roger Chalkley

doi:10.1093/nar/2.10.1751

An approach to histone nearest neighbours in extended chromatin

Ross C. Hardison, Mary Ellen Eichner, Roger Chalkley

Biochemistry & Molecular Biology

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28 Scopus citations

Abstract

The primary sequence organization of histones upon the DNA molecule in chromatin has been analyzed by extension of the nucleoprotein at very low ionic strength and crosslinking with a reversible crosslinking reagent, methyl-4-mercaptobutyrimidate. Histones extracted after limited reaction were fractionated into different classes and the composition of the oligomers analyzed after reduction of the crosslinked material. We have found that the following dimers occur at a high frequency: (F3-F2b), (F3-F2a2), and (F2b-F2a2), whereas (F2b-F2a1), (F3-F2a1) and (F3-F3) occur with a lower frequency. F1 appears to polymerize rapidly to largely homogeneous polymers of high molecular weight. These results are analyzed in terms of several models proposed for chromatin structure.

Original language	English (US)
Pages (from-to)	1751-1770
Number of pages	20
Journal	Nucleic acids research
Volume	2
Issue number	10
DOIs	https://doi.org/10.1093/nar/2.10.1751
State	Published - Oct 1975

All Science Journal Classification (ASJC) codes

Genetics

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10.1093/nar/2.10.1751

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title = "An approach to histone nearest neighbours in extended chromatin",

abstract = "The primary sequence organization of histones upon the DNA molecule in chromatin has been analyzed by extension of the nucleoprotein at very low ionic strength and crosslinking with a reversible crosslinking reagent, methyl-4-mercaptobutyrimidate. Histones extracted after limited reaction were fractionated into different classes and the composition of the oligomers analyzed after reduction of the crosslinked material. We have found that the following dimers occur at a high frequency: (F3-F2b), (F3-F2a2), and (F2b-F2a2), whereas (F2b-F2a1), (F3-F2a1) and (F3-F3) occur with a lower frequency. F1 appears to polymerize rapidly to largely homogeneous polymers of high molecular weight. These results are analyzed in terms of several models proposed for chromatin structure.",

author = "Hardison, {Ross C.} and Eichner, {Mary Ellen} and Roger Chalkley",

note = "Funding Information: This work was supported by USPHS grant CA 10871 and NIH predoctoral traineeship GM 550. We thank Debbie Parris, Vaughn Jackson, and Peter Hoffmann for assistance and helpful discussions, and Dr. Charles Swenson for use of his DuPont Curve Analyzer. We also thank the members of this laboratory for providing an efficient, mellow, and stimulating environment in which to work. REFERENCES",

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doi = "10.1093/nar/2.10.1751",

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AU - Hardison, Ross C.

AU - Eichner, Mary Ellen

AU - Chalkley, Roger

N1 - Funding Information: This work was supported by USPHS grant CA 10871 and NIH predoctoral traineeship GM 550. We thank Debbie Parris, Vaughn Jackson, and Peter Hoffmann for assistance and helpful discussions, and Dr. Charles Swenson for use of his DuPont Curve Analyzer. We also thank the members of this laboratory for providing an efficient, mellow, and stimulating environment in which to work. REFERENCES

PY - 1975/10

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N2 - The primary sequence organization of histones upon the DNA molecule in chromatin has been analyzed by extension of the nucleoprotein at very low ionic strength and crosslinking with a reversible crosslinking reagent, methyl-4-mercaptobutyrimidate. Histones extracted after limited reaction were fractionated into different classes and the composition of the oligomers analyzed after reduction of the crosslinked material. We have found that the following dimers occur at a high frequency: (F3-F2b), (F3-F2a2), and (F2b-F2a2), whereas (F2b-F2a1), (F3-F2a1) and (F3-F3) occur with a lower frequency. F1 appears to polymerize rapidly to largely homogeneous polymers of high molecular weight. These results are analyzed in terms of several models proposed for chromatin structure.

AB - The primary sequence organization of histones upon the DNA molecule in chromatin has been analyzed by extension of the nucleoprotein at very low ionic strength and crosslinking with a reversible crosslinking reagent, methyl-4-mercaptobutyrimidate. Histones extracted after limited reaction were fractionated into different classes and the composition of the oligomers analyzed after reduction of the crosslinked material. We have found that the following dimers occur at a high frequency: (F3-F2b), (F3-F2a2), and (F2b-F2a2), whereas (F2b-F2a1), (F3-F2a1) and (F3-F3) occur with a lower frequency. F1 appears to polymerize rapidly to largely homogeneous polymers of high molecular weight. These results are analyzed in terms of several models proposed for chromatin structure.

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An approach to histone nearest neighbours in extended chromatin

Abstract

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