TY - JOUR
T1 - An efficient method for measuring plasma volume using indocyanine green dye
AU - Aguree, Sixtus
AU - Gernand, Alison D.
N1 - Funding Information:
We thank the medical staff, especially Cyndi Flanagan, at the Clinical Research Center of the Pennsylvania State University’s Clinical and Translational Science Institute. We also extend our gratitude to Dr. James A. Pawelczyk, Associate Professor of Physiology and Kinesiology at The Pennsylvania State University, for his support in the study design, particularly on the use of indocyanine green. The authors sincerely acknowledge the research support provided by several research assistants, particularly, Leigh A. Taylor. We also thank the volunteers for their participation in the study. The project described was supported by the National Center for Advancing Translational Sciences, National Institutes of Health, through Grant UL1 TR002014. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH.
Funding Information:
The Pennsylvania State University, College of Health and Human Development. We thank the medical staff, especially Cyndi Flanagan, at the Clinical Research Center of the Pennsylvania State University's Clinical and Translational Science Institute. We also extend our gratitude to Dr. James A. Pawelczyk, Associate Professor of Physiology and Kinesiology at The Pennsylvania State University, for his support in the study design, particularly on the use of indocyanine green. The authors sincerely acknowledge the research support provided by several research assistants, particularly, Leigh A. Taylor. We also thank the volunteers for their participation in the study. The project described was supported by the National Center for Advancing Translational Sciences, National Institutes of Health, through Grant UL1 TR002014. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH.
Publisher Copyright:
© 2019 The Author(s)
PY - 2019
Y1 - 2019
N2 - Plasma volume (PV) can be an important marker of health status and may affect the interpretation of plasma biomarkers, but is rarely measured due to the complexity and time required. Indocyanine green (ICG) is a water-soluble tricarbocyanine dye with a circulatory half-life of 2–3 min, allowing for quick clearance and repeated use. It is used extensively in medical diagnostic tests including ophthalmologic imaging, liver function, and cardiac output, particularly in critical care. ICG has been validated for measuring PV in humans, however previous work has provided minimal published details or has focused on a single aspect of the method. We aimed to develop a detailed, optimal protocol for the use of ICG to measure PV in women of reproductive age. We combined best practices from other studies and optimized the protocol for efficiency. • This method reduces the time from blood collection to PV determination to ˜2 h and the amount of plasma required to estimate PV to 2.5 mL (1.5 mL before ICG injection and 1.0 mL post-injection). • Participant inconvenience is reduced by inserting an intravenous (IV) catheter in only one arm, not both arms. • Five post-injection plasma samples (2–5 min after ICG bolus) are enough to accurately develop the decay curve for plasma ICG concentration and estimate PV by extrapolation.
AB - Plasma volume (PV) can be an important marker of health status and may affect the interpretation of plasma biomarkers, but is rarely measured due to the complexity and time required. Indocyanine green (ICG) is a water-soluble tricarbocyanine dye with a circulatory half-life of 2–3 min, allowing for quick clearance and repeated use. It is used extensively in medical diagnostic tests including ophthalmologic imaging, liver function, and cardiac output, particularly in critical care. ICG has been validated for measuring PV in humans, however previous work has provided minimal published details or has focused on a single aspect of the method. We aimed to develop a detailed, optimal protocol for the use of ICG to measure PV in women of reproductive age. We combined best practices from other studies and optimized the protocol for efficiency. • This method reduces the time from blood collection to PV determination to ˜2 h and the amount of plasma required to estimate PV to 2.5 mL (1.5 mL before ICG injection and 1.0 mL post-injection). • Participant inconvenience is reduced by inserting an intravenous (IV) catheter in only one arm, not both arms. • Five post-injection plasma samples (2–5 min after ICG bolus) are enough to accurately develop the decay curve for plasma ICG concentration and estimate PV by extrapolation.
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U2 - 10.1016/j.mex.2019.05.003
DO - 10.1016/j.mex.2019.05.003
M3 - Article
C2 - 31193322
AN - SCOPUS:85065726286
SN - 2215-0161
VL - 6
SP - 1072
EP - 1083
JO - MethodsX
JF - MethodsX
ER -