An Evaluation of Analytical Methods for the Detection of Secondary Oxidation Products in Dedifferentiated Plant Cultures

D. H. Bremner; W. J. Magill; N. Deighton; E. E. Benson

An Evaluation of Analytical Methods for the Detection of Secondary Oxidation Products in Dedifferentiated Plant Cultures

D. H. Bremner, W. J. Magill, N. Deighton, E. E. Benson

Huck Institutes of the Life Sciences

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

A range of techniques have been employed to detect and examine aldehydic products of lipid peroxidation in D. carota cultures. Emphasis is placed on the use of HPLC and LC-MS techniques to aid the identification and profiling of aldehydic dinitrophenyl hydrazone (DNPH)-derivatives in dedifferentiated callus tissues. Using these approaches, malondialdehyde (MDA) and 4-hydroxynonenal (HNE) have been identified in cultures of D. carota. Using LC-MS, it was found that embryogenic cultures have high levels of HNE and lower levels of MDA compared to non-embryogenic cultures. Future studies will evaluate the role of these lipid peroxidation products in in vitro morphogenesis.

Original language	English (US)
Pages (from-to)	39-44
Number of pages	6
Journal	Phyton - Annales Rei Botanicae
Volume	37
Issue number	3
State	Published - Jan 7 1997

All Science Journal Classification (ASJC) codes

Global and Planetary Change
Physiology
Ecology, Evolution, Behavior and Systematics
Plant Science

Cite this

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title = "An Evaluation of Analytical Methods for the Detection of Secondary Oxidation Products in Dedifferentiated Plant Cultures",

abstract = "A range of techniques have been employed to detect and examine aldehydic products of lipid peroxidation in D. carota cultures. Emphasis is placed on the use of HPLC and LC-MS techniques to aid the identification and profiling of aldehydic dinitrophenyl hydrazone (DNPH)-derivatives in dedifferentiated callus tissues. Using these approaches, malondialdehyde (MDA) and 4-hydroxynonenal (HNE) have been identified in cultures of D. carota. Using LC-MS, it was found that embryogenic cultures have high levels of HNE and lower levels of MDA compared to non-embryogenic cultures. Future studies will evaluate the role of these lipid peroxidation products in in vitro morphogenesis.",

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AU - Deighton, N.

AU - Benson, E. E.

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N2 - A range of techniques have been employed to detect and examine aldehydic products of lipid peroxidation in D. carota cultures. Emphasis is placed on the use of HPLC and LC-MS techniques to aid the identification and profiling of aldehydic dinitrophenyl hydrazone (DNPH)-derivatives in dedifferentiated callus tissues. Using these approaches, malondialdehyde (MDA) and 4-hydroxynonenal (HNE) have been identified in cultures of D. carota. Using LC-MS, it was found that embryogenic cultures have high levels of HNE and lower levels of MDA compared to non-embryogenic cultures. Future studies will evaluate the role of these lipid peroxidation products in in vitro morphogenesis.

AB - A range of techniques have been employed to detect and examine aldehydic products of lipid peroxidation in D. carota cultures. Emphasis is placed on the use of HPLC and LC-MS techniques to aid the identification and profiling of aldehydic dinitrophenyl hydrazone (DNPH)-derivatives in dedifferentiated callus tissues. Using these approaches, malondialdehyde (MDA) and 4-hydroxynonenal (HNE) have been identified in cultures of D. carota. Using LC-MS, it was found that embryogenic cultures have high levels of HNE and lower levels of MDA compared to non-embryogenic cultures. Future studies will evaluate the role of these lipid peroxidation products in in vitro morphogenesis.

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An Evaluation of Analytical Methods for the Detection of Secondary Oxidation Products in Dedifferentiated Plant Cultures

Abstract

All Science Journal Classification (ASJC) codes

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