Abstract
A range of techniques have been employed to detect and examine aldehydic products of lipid peroxidation in D. carota cultures. Emphasis is placed on the use of HPLC and LC-MS techniques to aid the identification and profiling of aldehydic dinitrophenyl hydrazone (DNPH)-derivatives in dedifferentiated callus tissues. Using these approaches, malondialdehyde (MDA) and 4-hydroxynonenal (HNE) have been identified in cultures of D. carota. Using LC-MS, it was found that embryogenic cultures have high levels of HNE and lower levels of MDA compared to non-embryogenic cultures. Future studies will evaluate the role of these lipid peroxidation products in in vitro morphogenesis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 39-44 |
| Number of pages | 6 |
| Journal | Phyton - Annales Rei Botanicae |
| Volume | 37 |
| Issue number | 3 |
| State | Published - Jan 7 1997 |
All Science Journal Classification (ASJC) codes
- Global and Planetary Change
- Physiology
- Ecology, Evolution, Behavior and Systematics
- Plant Science