An improved method for isolating RNA from porcine adipose tissue

I. Louveau; S. Chaudhuri; T. D. Etherton

doi:10.1016/0003-2697(91)90471-5

An improved method for isolating RNA from porcine adipose tissue

I. Louveau
, S. Chaudhuri
, T. D. Etherton

Animal Science

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

In the present study, we describe a method that we developed to isolate total RNA from porcine adipose tissue. This method entails homogenizing porcine adipose tissue in 10 ml of 4 m guanidium thiocyanate, 25 mm sodium citrate, 0.5% Sarcosyl, 0.1 m β-mercaptoethanol, pH 7.0, and then performing two CHCl₃ extractions to remove lipid before following the procedure described by P. Chomczynski and N. Sacchi (1987, Anal. Biochem. 162, 156-159). This modification improved the yield of RNA approximately threefold (yield was 88 ± 7 μg total RNA/g of tissue) without affecting RNA quality.

Original language	English (US)
Pages (from-to)	308-310
Number of pages	3
Journal	Analytical Biochemistry
Volume	196
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(91)90471-5
State	Published - Aug 1 1991

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(91)90471-5

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abstract = "In the present study, we describe a method that we developed to isolate total RNA from porcine adipose tissue. This method entails homogenizing porcine adipose tissue in 10 ml of 4 m guanidium thiocyanate, 25 mm sodium citrate, 0.5\% Sarcosyl, 0.1 m β-mercaptoethanol, pH 7.0, and then performing two CHCl3 extractions to remove lipid before following the procedure described by P. Chomczynski and N. Sacchi (1987, Anal. Biochem. 162, 156-159). This modification improved the yield of RNA approximately threefold (yield was 88 ± 7 μg total RNA/g of tissue) without affecting RNA quality.",

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N2 - In the present study, we describe a method that we developed to isolate total RNA from porcine adipose tissue. This method entails homogenizing porcine adipose tissue in 10 ml of 4 m guanidium thiocyanate, 25 mm sodium citrate, 0.5% Sarcosyl, 0.1 m β-mercaptoethanol, pH 7.0, and then performing two CHCl3 extractions to remove lipid before following the procedure described by P. Chomczynski and N. Sacchi (1987, Anal. Biochem. 162, 156-159). This modification improved the yield of RNA approximately threefold (yield was 88 ± 7 μg total RNA/g of tissue) without affecting RNA quality.

AB - In the present study, we describe a method that we developed to isolate total RNA from porcine adipose tissue. This method entails homogenizing porcine adipose tissue in 10 ml of 4 m guanidium thiocyanate, 25 mm sodium citrate, 0.5% Sarcosyl, 0.1 m β-mercaptoethanol, pH 7.0, and then performing two CHCl3 extractions to remove lipid before following the procedure described by P. Chomczynski and N. Sacchi (1987, Anal. Biochem. 162, 156-159). This modification improved the yield of RNA approximately threefold (yield was 88 ± 7 μg total RNA/g of tissue) without affecting RNA quality.

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An improved method for isolating RNA from porcine adipose tissue

Abstract

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