Abstract
We propose a phosphodiesterase assay based on 1D 1H NMR to monitor the hydrolysis of cyclic nucleotides directly, without requiring tags or the addition of exogenous reagents. The method is suitable to measure phosphodiesterase KM and kcat parameters and to identify phosphodiesterase inhibitors.
Original language | English (US) |
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Pages (from-to) | 8091-8094 |
Number of pages | 4 |
Journal | Chemical Communications |
Volume | 56 |
Issue number | 58 |
DOIs | |
State | Published - Jul 25 2020 |
All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Catalysis
- Ceramics and Composites
- General Chemistry
- Surfaces, Coatings and Films
- Metals and Alloys
- Materials Chemistry