An on-farm bioassay for assessing Meloidogyne hapla infestations as a decision management tool

B. K. Gugino; J. W. Ludwig; G. S. Abawi

doi:10.1016/j.cropro.2007.11.004

An on-farm bioassay for assessing Meloidogyne hapla infestations as a decision management tool

B. K. Gugino, J. W. Ludwig, G. S. Abawi

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

A simple, visual soil bioassay was developed to assess Meloidogyne hapla soil infestation levels in order to facilitate the implementation of management practices on an as-needed basis. Generally, four composite soil samples were collected per target field, planted with lettuce (Lactuca sativa) seedlings and maintained in a greenhouse for this study. After 4-6 weeks, the root systems were washed free of soil and scored for root-galling severity by counting the total number of galls that developed or estimating the percentage of root system galled. Crop-specific thresholds or the presence or absence of galls are then used in part to make M. hapla management decisions. This bioassay was simplified and adapted for on-farm use by growers and has already been successfully utilized in making nematode management decisions.

Original language	English (US)
Pages (from-to)	785-791
Number of pages	7
Journal	Crop Protection
Volume	27
Issue number	3-5
DOIs	https://doi.org/10.1016/j.cropro.2007.11.004
State	Published - Mar 2008

All Science Journal Classification (ASJC) codes

Agronomy and Crop Science

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10.1016/j.cropro.2007.11.004

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@article{22c43a96a8364a438ea12161c3545cce,

title = "An on-farm bioassay for assessing Meloidogyne hapla infestations as a decision management tool",

abstract = "A simple, visual soil bioassay was developed to assess Meloidogyne hapla soil infestation levels in order to facilitate the implementation of management practices on an as-needed basis. Generally, four composite soil samples were collected per target field, planted with lettuce (Lactuca sativa) seedlings and maintained in a greenhouse for this study. After 4-6 weeks, the root systems were washed free of soil and scored for root-galling severity by counting the total number of galls that developed or estimating the percentage of root system galled. Crop-specific thresholds or the presence or absence of galls are then used in part to make M. hapla management decisions. This bioassay was simplified and adapted for on-farm use by growers and has already been successfully utilized in making nematode management decisions.",

author = "Gugino, {B. K.} and Ludwig, {J. W.} and Abawi, {G. S.}",

note = "Funding Information: The authors thank J. Barnard for statistical assistance, C.R. MacNeil, C.A. Hoepting, J. van der Heide, and M.R. Ulrich of Cornell Cooperative Extension for their assistance is the selection and sampling of commercial grower sites over the years and numerous collaborating growers for their interest and cooperation. This work was in part supported by New York State Onion Research and Development Program, the Multi-State Regional Nematology Project NE-1019, and New York State Integrated Pest Management.",

year = "2008",

month = mar,

doi = "10.1016/j.cropro.2007.11.004",

language = "English (US)",

volume = "27",

pages = "785--791",

journal = "Crop Protection",

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publisher = "Elsevier B.V.",

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AU - Gugino, B. K.

AU - Ludwig, J. W.

AU - Abawi, G. S.

N1 - Funding Information: The authors thank J. Barnard for statistical assistance, C.R. MacNeil, C.A. Hoepting, J. van der Heide, and M.R. Ulrich of Cornell Cooperative Extension for their assistance is the selection and sampling of commercial grower sites over the years and numerous collaborating growers for their interest and cooperation. This work was in part supported by New York State Onion Research and Development Program, the Multi-State Regional Nematology Project NE-1019, and New York State Integrated Pest Management.

PY - 2008/3

Y1 - 2008/3

N2 - A simple, visual soil bioassay was developed to assess Meloidogyne hapla soil infestation levels in order to facilitate the implementation of management practices on an as-needed basis. Generally, four composite soil samples were collected per target field, planted with lettuce (Lactuca sativa) seedlings and maintained in a greenhouse for this study. After 4-6 weeks, the root systems were washed free of soil and scored for root-galling severity by counting the total number of galls that developed or estimating the percentage of root system galled. Crop-specific thresholds or the presence or absence of galls are then used in part to make M. hapla management decisions. This bioassay was simplified and adapted for on-farm use by growers and has already been successfully utilized in making nematode management decisions.

AB - A simple, visual soil bioassay was developed to assess Meloidogyne hapla soil infestation levels in order to facilitate the implementation of management practices on an as-needed basis. Generally, four composite soil samples were collected per target field, planted with lettuce (Lactuca sativa) seedlings and maintained in a greenhouse for this study. After 4-6 weeks, the root systems were washed free of soil and scored for root-galling severity by counting the total number of galls that developed or estimating the percentage of root system galled. Crop-specific thresholds or the presence or absence of galls are then used in part to make M. hapla management decisions. This bioassay was simplified and adapted for on-farm use by growers and has already been successfully utilized in making nematode management decisions.

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An on-farm bioassay for assessing Meloidogyne hapla infestations as a decision management tool

Abstract

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