An optimized approach to germ-free rearing in the jewel wasp Nasonia

J. Dylan Shropshire, Edward J. van Opstal, Seth R. Bordenstein

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


Development of a Nasonia in vitro germ-free rearing system in 2012 enabled investigation of Nasonia-microbiota interactions and real-time visualization of parasitoid metamorphosis. However, the use of antibiotics, bleach, and fetal bovine serum introduced artifacts relative to conventional rearing of Nasonia. Here, we optimize the germ-free rearing procedure by using filter sterilization in lieu of antibiotics and by removing residual bleach and fetal bovine serum. Comparison of these methods reveals no influence on larval survival or growth, and a 52% improvement in adult production. Additionally, adult males produced in the new germ-free system are similar in size to conventionally reared males. Experimental implications of these changes are discussed.

Original languageEnglish (US)
Article numbere2316
Issue number9
StatePublished - 2016

All Science Journal Classification (ASJC) codes

  • General Neuroscience
  • General Biochemistry, Genetics and Molecular Biology
  • General Agricultural and Biological Sciences


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