An optimized protocol for the generation of HBV viral antigen-specific T lymphocytes from pluripotent stem cells

Mohammad Haque, Xiaofang Xiong, Fengyang Lei, Jugal Kishore Das, Jianxun Song

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

In T cell-based cancer immunotherapy, tumor antigen (Ag)-specific CD8+ cytotoxic T lymphocytes (CTLs) can specifically target tumor Ags on malignant cells. This promising approach drove us to adopt this strategy of T cell transfer (ACT)-based immunotherapy for chronic viral infections. Here, we describe the generation of hepatitis B virus (HBV) Ag-specific CTLs from induced pluripotent stem cells (iPSCs), i.e., iPSC-CTLs. Ag-specific iPSC-CTLs can target HBV Ag+ cells and infiltrate into the liver to suppress HBV replication in a murine model. For complete details on the use and execution of this protocol, please refer to Haque et al. (2020).

Original languageEnglish (US)
Article number100264
JournalSTAR Protocols
Volume2
Issue number1
DOIs
StatePublished - Mar 19 2021

All Science Journal Classification (ASJC) codes

  • General Biochemistry, Genetics and Molecular Biology
  • General Neuroscience
  • General Immunology and Microbiology
  • General Medicine

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