@article{ea77816b64184df7b09506b7fcc7ba32,
title = "An optimized protocol for the generation of HBV viral antigen-specific T lymphocytes from pluripotent stem cells",
abstract = "In T cell-based cancer immunotherapy, tumor antigen (Ag)-specific CD8+ cytotoxic T lymphocytes (CTLs) can specifically target tumor Ags on malignant cells. This promising approach drove us to adopt this strategy of T cell transfer (ACT)-based immunotherapy for chronic viral infections. Here, we describe the generation of hepatitis B virus (HBV) Ag-specific CTLs from induced pluripotent stem cells (iPSCs), i.e., iPSC-CTLs. Ag-specific iPSC-CTLs can target HBV Ag+ cells and infiltrate into the liver to suppress HBV replication in a murine model. For complete details on the use and execution of this protocol, please refer to Haque et al. (2020).",
author = "Mohammad Haque and Xiaofang Xiong and Fengyang Lei and Das, {Jugal Kishore} and Jianxun Song",
note = "Funding Information: The authors thank Dr. Pei-Jer Chen from National Taiwan University Hospital for providing the pAAV/HBV1.2 construct and Dr. Adam J. Gehring from Toronto General Hospital Research Institute for providing the s183-specific TCR genes. This work was supported by the National Institutes of Health grants R01AI121180, R01CA221867, and R21AI128325 to J.S. J.S. designed the experiments, analyzed data, and contributed to the writing of the paper. M.H. F.L. and J.K.D. performed the experiments. X.X. and M.H. analyzed data. The authors declare no competing interests. Funding Information: The authors thank Dr. Pei-Jer Chen from National Taiwan University Hospital for providing the pAAV/HBV1.2 construct and Dr. Adam J. Gehring from Toronto General Hospital Research Institute for providing the s183-specific TCR genes. This work was supported by the National Institutes of Health grants R01AI121180 , R01CA221867 , and R21AI128325 to J.S. Publisher Copyright: {\textcopyright} 2020 The Author(s)",
year = "2021",
month = mar,
day = "19",
doi = "10.1016/j.xpro.2020.100264",
language = "English (US)",
volume = "2",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "1",
}
