TY - JOUR
T1 - An SV40 VP1-derived epitope recognized by CD8+ T cells is naturally processed and presented by HLA-A*0201 and cross-reactive with human polyomavirus determinants
AU - Tagaram, Hephzibah Rani S.
AU - Watson, Alan M.
AU - Lemonnier, Francois A.
AU - Staveley-O'Carroll, Kevin
AU - Tevethia, Satvir S.
AU - Schell, Todd D.
N1 - Funding Information:
We thank Jeremy Haley for excellent technical assistance and Nate Schaffer for assistance in the Flow Cytometry Core Facility. This work was supported by research grants CA-25000 from the National Cancer Institute/National Institutes of Health, a grant from the Barsumian Trust and a grant from the Pennsylvania Tobacco Settlement. KSOC was supported by KO8 award #416-67HY from the National Institutes of Health.
PY - 2008/6/20
Y1 - 2008/6/20
N2 - The CD8+ T cell responses directed toward the VP1 antigens of human polyomaviruses JC and BK recently were shown to be cross-reactive. Two HLA-A*0201-restricted determinants from each virus have been defined and include JCp100-108 (ILMWEAVTL) and BKp108-116 (LLMWEAVTV) as well as JCp36-44 (SITEVECFL) and BKp44-52 (AITEVECFL). We asked whether VP1 from the related SV40 contains similar HLA-A*0201-restricted determinants. In this study, we demonstrate that CD8+ T cells specific for SV40 VP1 p110-118 (ILMWEAVTV), but not p46-54 (SFTEVECFL), can be induced in HLA-A*0201-transgenic mice and that these CD8+ T cells cross-react with the corresponding determinants from JC and BK virus. The SV40 p110 determinant was found to be processed and presented in SV40-infected cells. These results indicate that the JCp36/BKp44 determinants are distinctive for the human polyomaviruses while the JCp100/BKp108/SVp110 determinants are shared by all three viruses, providing a target for CD8+ T cell cross-reactivity.
AB - The CD8+ T cell responses directed toward the VP1 antigens of human polyomaviruses JC and BK recently were shown to be cross-reactive. Two HLA-A*0201-restricted determinants from each virus have been defined and include JCp100-108 (ILMWEAVTL) and BKp108-116 (LLMWEAVTV) as well as JCp36-44 (SITEVECFL) and BKp44-52 (AITEVECFL). We asked whether VP1 from the related SV40 contains similar HLA-A*0201-restricted determinants. In this study, we demonstrate that CD8+ T cells specific for SV40 VP1 p110-118 (ILMWEAVTV), but not p46-54 (SFTEVECFL), can be induced in HLA-A*0201-transgenic mice and that these CD8+ T cells cross-react with the corresponding determinants from JC and BK virus. The SV40 p110 determinant was found to be processed and presented in SV40-infected cells. These results indicate that the JCp36/BKp44 determinants are distinctive for the human polyomaviruses while the JCp100/BKp108/SVp110 determinants are shared by all three viruses, providing a target for CD8+ T cell cross-reactivity.
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U2 - 10.1016/j.virol.2008.02.033
DO - 10.1016/j.virol.2008.02.033
M3 - Article
C2 - 18402997
AN - SCOPUS:43749111973
SN - 0042-6822
VL - 376
SP - 183
EP - 190
JO - Virology
JF - Virology
IS - 1
ER -