An Ultrafast One-Step Quantitative Reverse Transcription–Polymerase Chain Reaction Assay for Detection of SARS-CoV-2

Jadranka Milosevic, Mengrou Lu, Wallace Greene, Hong Zhang He, Si Yang Zheng

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

We developed an ultrafast one-step RT-qPCR assay for SARS-CoV-2 detection, which can be completed in only 30 min on benchtop Bio-Rad CFX96. The assay significantly reduces the running time of conventional RT-qPCR: reduced RT step from 10 to 1 min, and reduced the PCR cycle of denaturation from 10 to 1 s and extension from 30 to 1 s. A cohort of 60 nasopharyngeal swab samples testing showed that the assay had a clinical sensitivity of 100% and a clinical specificity of 100%.

Original languageEnglish (US)
Article number749783
JournalFrontiers in Microbiology
Volume12
DOIs
StatePublished - Nov 4 2021

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)

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