An Ultrafast One-Step Quantitative Reverse Transcription–Polymerase Chain Reaction Assay for Detection of SARS-CoV-2

Jadranka Milosevic; Mengrou Lu; Wallace Greene; Hong Zhang He; Si Yang Zheng

doi:10.3389/fmicb.2021.749783

An Ultrafast One-Step Quantitative Reverse Transcription–Polymerase Chain Reaction Assay for Detection of SARS-CoV-2

Jadranka Milosevic
, Mengrou Lu
, Wallace Greene
, Hong Zhang He
, Si Yang Zheng

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

We developed an ultrafast one-step RT-qPCR assay for SARS-CoV-2 detection, which can be completed in only 30 min on benchtop Bio-Rad CFX96. The assay significantly reduces the running time of conventional RT-qPCR: reduced RT step from 10 to 1 min, and reduced the PCR cycle of denaturation from 10 to 1 s and extension from 30 to 1 s. A cohort of 60 nasopharyngeal swab samples testing showed that the assay had a clinical sensitivity of 100% and a clinical specificity of 100%.

Original language	English (US)
Article number	749783
Journal	Frontiers in Microbiology
Volume	12
DOIs	https://doi.org/10.3389/fmicb.2021.749783
State	Published - Nov 4 2021

All Science Journal Classification (ASJC) codes

Microbiology
Microbiology (medical)

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title = "An Ultrafast One-Step Quantitative Reverse Transcription–Polymerase Chain Reaction Assay for Detection of SARS-CoV-2",

abstract = "We developed an ultrafast one-step RT-qPCR assay for SARS-CoV-2 detection, which can be completed in only 30 min on benchtop Bio-Rad CFX96. The assay significantly reduces the running time of conventional RT-qPCR: reduced RT step from 10 to 1 min, and reduced the PCR cycle of denaturation from 10 to 1 s and extension from 30 to 1 s. A cohort of 60 nasopharyngeal swab samples testing showed that the assay had a clinical sensitivity of 100\% and a clinical specificity of 100\%.",

author = "Jadranka Milosevic and Mengrou Lu and Wallace Greene and He, \{Hong Zhang\} and Zheng, \{Si Yang\}",

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AU - Lu, Mengrou

AU - Greene, Wallace

AU - He, Hong Zhang

AU - Zheng, Si Yang

PY - 2021/11/4

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AB - We developed an ultrafast one-step RT-qPCR assay for SARS-CoV-2 detection, which can be completed in only 30 min on benchtop Bio-Rad CFX96. The assay significantly reduces the running time of conventional RT-qPCR: reduced RT step from 10 to 1 min, and reduced the PCR cycle of denaturation from 10 to 1 s and extension from 30 to 1 s. A cohort of 60 nasopharyngeal swab samples testing showed that the assay had a clinical sensitivity of 100% and a clinical specificity of 100%.

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UR - https://www.scopus.com/pages/publications/85119441370#tab=citedBy

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An Ultrafast One-Step Quantitative Reverse Transcription–Polymerase Chain Reaction Assay for Detection of SARS-CoV-2

Abstract

All Science Journal Classification (ASJC) codes

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