Analysis and purification of mouse intestinal dendritic cell and macrophage subsets by flow cytometry

Balázs Koscsó; Milena Bogunovic

doi:10.1002/cpim.11

Analysis and purification of mouse intestinal dendritic cell and macrophage subsets by flow cytometry

Balázs Koscsó, Milena Bogunovic

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7 Scopus citations

Abstract

The unit presents a method for analysis of intestinal dendritic cell (DC) and macrophage subsets by flow cytometry in the single cell suspension prepared from the mouse small and large intestine (Basic Protocol). Alternate Protocol 1 describes a strategy to enrich the hematopoietic cell fraction in the sample by Percoll gradient centrifugation, and Alternate Protocol 2 describes preparation of single cell suspensions from specific tissue layers of the small intestine, such as the epithelium, villi mucosa, submucosa, and muscularis externa. Finally, Support Protocol explains how to purify specific intestinal DC and macrophage subsets by flow-cytometry-based cell sorting.

Original language	English (US)
Pages (from-to)	14.39.1-14.39.14
Journal	Current Protocols in Immunology
Volume	2016
DOIs	https://doi.org/10.1002/cpim.11
State	Published - 2016

All Science Journal Classification (ASJC) codes

Immunology

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10.1002/cpim.11

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year = "2016",

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AB - The unit presents a method for analysis of intestinal dendritic cell (DC) and macrophage subsets by flow cytometry in the single cell suspension prepared from the mouse small and large intestine (Basic Protocol). Alternate Protocol 1 describes a strategy to enrich the hematopoietic cell fraction in the sample by Percoll gradient centrifugation, and Alternate Protocol 2 describes preparation of single cell suspensions from specific tissue layers of the small intestine, such as the epithelium, villi mucosa, submucosa, and muscularis externa. Finally, Support Protocol explains how to purify specific intestinal DC and macrophage subsets by flow-cytometry-based cell sorting.

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JO - Current Protocols in Immunology

JF - Current Protocols in Immunology

ER -

Analysis and purification of mouse intestinal dendritic cell and macrophage subsets by flow cytometry

Abstract

All Science Journal Classification (ASJC) codes

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