TY - JOUR
T1 - Analysis of Benzoquinolines and Acridines in a Brazilian Diesel Oil by Particle Beam LC/MS and HPLC/UV
AU - Mao, John
AU - Pacheco, Carlos R.
AU - Traficante, Daniel D.
AU - Rosen, William
N1 - Funding Information:
The authors would like to thank Petrobras for providing the diesel oil samples. This project was funded by Petrobras, Springborn Laboratories, Inc., and NMR Concepts as a cooperative venture.
PY - 1995/3/1
Y1 - 1995/3/1
N2 - Nitrogen bases were isolated from a Brazilian diesel distillate by acid extraction. With minimum sample preparation and clean-up, nitrogen bases were analyzed by particle beam LC/MS and HPLC with photo diode-array detection using reversed phase chromatography. Benzoquinolines were identified as the major nitrogen containing compounds in this basic fraction. By using neutral mobile phases, benzoquinoline homologues were separated, enabling rapid class characterization as well as preparative HPLC isolation of individual benzoquinoline homologues. Acidified mobile phases, however, exhibited greater resolution for individual isomers. UV spectroscopy was used to differentiate various types of benzoquinolines (e.g, benzo[f]quinolines, benzo[h]quinolines, and acridines). Acridines can be easily distinguished from benzofh]- or benzo[f]quinolines by its different UV absorption spectrum. Benzo[h]- and benzo[f]quinolines were differentiated by the differences in the first derivative of the absorbance (dA/dl) vs. wavelength. To confirm the identification, nitrogen bases were also analyzed by conventional GC/MS methods.
AB - Nitrogen bases were isolated from a Brazilian diesel distillate by acid extraction. With minimum sample preparation and clean-up, nitrogen bases were analyzed by particle beam LC/MS and HPLC with photo diode-array detection using reversed phase chromatography. Benzoquinolines were identified as the major nitrogen containing compounds in this basic fraction. By using neutral mobile phases, benzoquinoline homologues were separated, enabling rapid class characterization as well as preparative HPLC isolation of individual benzoquinoline homologues. Acidified mobile phases, however, exhibited greater resolution for individual isomers. UV spectroscopy was used to differentiate various types of benzoquinolines (e.g, benzo[f]quinolines, benzo[h]quinolines, and acridines). Acridines can be easily distinguished from benzofh]- or benzo[f]quinolines by its different UV absorption spectrum. Benzo[h]- and benzo[f]quinolines were differentiated by the differences in the first derivative of the absorbance (dA/dl) vs. wavelength. To confirm the identification, nitrogen bases were also analyzed by conventional GC/MS methods.
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U2 - 10.1080/10826079508010401
DO - 10.1080/10826079508010401
M3 - Article
AN - SCOPUS:0028898717
SN - 0148-3919
VL - 18
SP - 903
EP - 916
JO - Journal of Liquid Chromatography
JF - Journal of Liquid Chromatography
IS - 5
ER -