TY - JOUR
T1 - Analysis of phospholipid molecular species by liquid chromatography - Atmospheric pressure chemical ionisation mass spectrometry of diacylglycerol nicotinates
AU - Dobson, Gary
AU - Deighton, Nigel
N1 - Funding Information:
This paper is published as part of a program funded by the Scottish Executive Rural Affairs Dept. We thank Y. Itabashi for providing standard diacylglycerols and W.W. Christie for helpful discussions.
PY - 2001
Y1 - 2001
N2 - A method using liquid chromatography - atmospheric pressure chemical ionisation mass spectrometry was evaluated for determining the molecular species composition of phospholipids (phosphatidylcholines from soybean, egg yolk and bovine liver) after conversion to diacylglycerol nicotinate derivatives. The structures could be deduced from pseudo-molecular ions ([MH-123]+) and three pairs of monoacyl containing fragment ions. All molecular species in mixed peaks were readily identified and many minor components, earlier not encountered in the samples under investigation, were identified. Acyl chain regioisomers were readily distinguished by the ratio of the [MH-RCHCO]+ ions. Molecular species differing only in the position of the double bonds in one polyunsaturated acyl chain were separated on the basis of retention times. A half quantitative estimation of the molecular species composition of complex samples was achieved by a combination of UV detection and, for mixed peaks, the areas of [MH-123]+ ions.
AB - A method using liquid chromatography - atmospheric pressure chemical ionisation mass spectrometry was evaluated for determining the molecular species composition of phospholipids (phosphatidylcholines from soybean, egg yolk and bovine liver) after conversion to diacylglycerol nicotinate derivatives. The structures could be deduced from pseudo-molecular ions ([MH-123]+) and three pairs of monoacyl containing fragment ions. All molecular species in mixed peaks were readily identified and many minor components, earlier not encountered in the samples under investigation, were identified. Acyl chain regioisomers were readily distinguished by the ratio of the [MH-RCHCO]+ ions. Molecular species differing only in the position of the double bonds in one polyunsaturated acyl chain were separated on the basis of retention times. A half quantitative estimation of the molecular species composition of complex samples was achieved by a combination of UV detection and, for mixed peaks, the areas of [MH-123]+ ions.
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U2 - 10.1016/S0009-3084(01)00130-X
DO - 10.1016/S0009-3084(01)00130-X
M3 - Article
C2 - 11438280
AN - SCOPUS:0034960546
SN - 0009-3084
VL - 111
SP - 1
EP - 17
JO - Chemistry and Physics of Lipids
JF - Chemistry and Physics of Lipids
IS - 1
ER -