Analysis of Single Cells by Capillary Electrophoresis with On-Column Derivatization and Laser-Induced Fluorescence Detection

S. Douglass Gilman; Andrew G. Ewing

doi:10.1021/ac00097a010

Analysis of Single Cells by Capillary Electrophoresis with On-Column Derivatization and Laser-Induced Fluorescence Detection

S. Douglass Gilman, Andrew G. Ewing

Chemistry

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205 Scopus citations

Abstract

On-column derivatization of single mammalian cells with capillary electrophoretic separation and laser-induced fluorescence detection is described. Individual cells are electrophorectically injected into the front end of the separation capillary, which is used as a chamber to lyse the cell and derivatize its contents for subsequent separation and detection. Reagents to lyse the cell and derivatize its contents are electrophoretically injected into the front end of the capillary (2.7 mm, 600-pL volume for a 17-µm -i.d. capillary) after the cell has been injected. Dopamine and five amino acids have been quantitatively determined in individual rat pheochromocytoma cells after on-column derivatization with naphthalene-2, 3-dicarboxaldehyde and CN^-. Average values of compounds determined in these cells range from 180 ±110 amol/ cell for aspartic acid to 5.1 ± 1.5 fmol/cell for taurine.

Original language	English (US)
Pages (from-to)	58-64
Number of pages	7
Journal	Analytical Chemistry
Volume	67
Issue number	1
DOIs	https://doi.org/10.1021/ac00097a010
State	Published - Jan 1 1995

All Science Journal Classification (ASJC) codes

Analytical Chemistry

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10.1021/ac00097a010

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title = "Analysis of Single Cells by Capillary Electrophoresis with On-Column Derivatization and Laser-Induced Fluorescence Detection",

abstract = "On-column derivatization of single mammalian cells with capillary electrophoretic separation and laser-induced fluorescence detection is described. Individual cells are electrophorectically injected into the front end of the separation capillary, which is used as a chamber to lyse the cell and derivatize its contents for subsequent separation and detection. Reagents to lyse the cell and derivatize its contents are electrophoretically injected into the front end of the capillary (2.7 mm, 600-pL volume for a 17-µm -i.d. capillary) after the cell has been injected. Dopamine and five amino acids have been quantitatively determined in individual rat pheochromocytoma cells after on-column derivatization with naphthalene-2, 3-dicarboxaldehyde and CN-. Average values of compounds determined in these cells range from 180 ±110 amol/ cell for aspartic acid to 5.1 ± 1.5 fmol/cell for taurine.",

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T1 - Analysis of Single Cells by Capillary Electrophoresis with On-Column Derivatization and Laser-Induced Fluorescence Detection

AU - Gilman, S. Douglass

AU - Ewing, Andrew G.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - On-column derivatization of single mammalian cells with capillary electrophoretic separation and laser-induced fluorescence detection is described. Individual cells are electrophorectically injected into the front end of the separation capillary, which is used as a chamber to lyse the cell and derivatize its contents for subsequent separation and detection. Reagents to lyse the cell and derivatize its contents are electrophoretically injected into the front end of the capillary (2.7 mm, 600-pL volume for a 17-µm -i.d. capillary) after the cell has been injected. Dopamine and five amino acids have been quantitatively determined in individual rat pheochromocytoma cells after on-column derivatization with naphthalene-2, 3-dicarboxaldehyde and CN-. Average values of compounds determined in these cells range from 180 ±110 amol/ cell for aspartic acid to 5.1 ± 1.5 fmol/cell for taurine.

AB - On-column derivatization of single mammalian cells with capillary electrophoretic separation and laser-induced fluorescence detection is described. Individual cells are electrophorectically injected into the front end of the separation capillary, which is used as a chamber to lyse the cell and derivatize its contents for subsequent separation and detection. Reagents to lyse the cell and derivatize its contents are electrophoretically injected into the front end of the capillary (2.7 mm, 600-pL volume for a 17-µm -i.d. capillary) after the cell has been injected. Dopamine and five amino acids have been quantitatively determined in individual rat pheochromocytoma cells after on-column derivatization with naphthalene-2, 3-dicarboxaldehyde and CN-. Average values of compounds determined in these cells range from 180 ±110 amol/ cell for aspartic acid to 5.1 ± 1.5 fmol/cell for taurine.

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Analysis of Single Cells by Capillary Electrophoresis with On-Column Derivatization and Laser-Induced Fluorescence Detection

Abstract

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