Analysis of single mammalian cells with capillary electrophoresis

Lori A. Woods; Andrew G. Ewing

doi:10.1007/s00216-003-1860-y

Analysis of single mammalian cells with capillary electrophoresis

Lori A. Woods, Andrew G. Ewing

Chemistry

Research output: Contribution to journal › Review article › peer-review

9 Scopus citations

Abstract

The investigation of single mammalian cells by using capillary electrophoresis (CE) is presented. CE allows to analyze single cell samples with volumes ranging from nanoliters to femtoliters. It provides fast and highly efficient separations of diverse analytes. It can also be coupled with various detection techniques to achieve sensitivity necessary for single cell analysis. Separations of proteins in single human colon adenocarcinoma cells have been achieved with CE and laser induced fluorescence (LIF) detection. In addition to injection techniques for the CE analysis of cell composition, sample injection techniques to monitor secretion from an individual mammalian cell with CE have also been developed.

Original language	English (US)
Pages (from-to)	281-283
Number of pages	3
Journal	Analytical and Bioanalytical Chemistry
Volume	376
Issue number	3
DOIs	https://doi.org/10.1007/s00216-003-1860-y
State	Published - Jun 2003

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Biochemistry

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title = "Analysis of single mammalian cells with capillary electrophoresis",

abstract = "The investigation of single mammalian cells by using capillary electrophoresis (CE) is presented. CE allows to analyze single cell samples with volumes ranging from nanoliters to femtoliters. It provides fast and highly efficient separations of diverse analytes. It can also be coupled with various detection techniques to achieve sensitivity necessary for single cell analysis. Separations of proteins in single human colon adenocarcinoma cells have been achieved with CE and laser induced fluorescence (LIF) detection. In addition to injection techniques for the CE analysis of cell composition, sample injection techniques to monitor secretion from an individual mammalian cell with CE have also been developed.",

author = "Woods, {Lori A.} and Ewing, {Andrew G.}",

note = "Funding Information: Acknowledgement The National Science Foundation and the National Institutes of Health are acknowledged for support of the authors{\textquoteright} work in this area.",

year = "2003",

month = jun,

doi = "10.1007/s00216-003-1860-y",

language = "English (US)",

volume = "376",

pages = "281--283",

journal = "Analytical and Bioanalytical Chemistry",

issn = "1618-2642",

publisher = "Springer Verlag",

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TY - JOUR

T1 - Analysis of single mammalian cells with capillary electrophoresis

AU - Woods, Lori A.

AU - Ewing, Andrew G.

N1 - Funding Information: Acknowledgement The National Science Foundation and the National Institutes of Health are acknowledged for support of the authors’ work in this area.

PY - 2003/6

Y1 - 2003/6

N2 - The investigation of single mammalian cells by using capillary electrophoresis (CE) is presented. CE allows to analyze single cell samples with volumes ranging from nanoliters to femtoliters. It provides fast and highly efficient separations of diverse analytes. It can also be coupled with various detection techniques to achieve sensitivity necessary for single cell analysis. Separations of proteins in single human colon adenocarcinoma cells have been achieved with CE and laser induced fluorescence (LIF) detection. In addition to injection techniques for the CE analysis of cell composition, sample injection techniques to monitor secretion from an individual mammalian cell with CE have also been developed.

AB - The investigation of single mammalian cells by using capillary electrophoresis (CE) is presented. CE allows to analyze single cell samples with volumes ranging from nanoliters to femtoliters. It provides fast and highly efficient separations of diverse analytes. It can also be coupled with various detection techniques to achieve sensitivity necessary for single cell analysis. Separations of proteins in single human colon adenocarcinoma cells have been achieved with CE and laser induced fluorescence (LIF) detection. In addition to injection techniques for the CE analysis of cell composition, sample injection techniques to monitor secretion from an individual mammalian cell with CE have also been developed.

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U2 - 10.1007/s00216-003-1860-y

DO - 10.1007/s00216-003-1860-y

M3 - Review article

C2 - 12774183

AN - SCOPUS:0041419661

SN - 1618-2642

VL - 376

SP - 281

EP - 283

JO - Analytical and Bioanalytical Chemistry

JF - Analytical and Bioanalytical Chemistry

IS - 3

ER -

Analysis of single mammalian cells with capillary electrophoresis

Abstract

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