TY - JOUR
T1 - Analysis of the CO dehydrogenase/acetyl-coenzyme A synthase operon of Mmethanosarcina thermophila
AU - Maupin-Furlow, Julie A.
AU - Ferry, James G.
PY - 1996
Y1 - 1996
N2 - The cdhABC genes encoding the respective α, ε, and β subunits of the five-subunit (α, β, γ, δ, and ε) CO dehydrogenase/acetyl-coenzyme synthase (CODH/ACS) complex from Methanosarcina thermophila were cloned and sequenced. Northern (RNA) blot analyses indicated that the cdh genes encoding the five subunits and an open reading frame (ORF1) with unknown function are cotranscribed during growth on acetate. Northern blot and primer extension analyses suggested that mRNA processing and multiple promoters may be involved in cdh transcript synthesis. The putative CdhA (α subunit) and CdhB (ε subunit) proteins each have 40% identity to CdhA and CdhB of the CODH/ACS complex from Methanosaeta soehngenii. The cdhC gene encodes the subunit (CdhC) of the CODH/ACS complex from M. thermophila. The N-terminal 397 amino acids of CdhC are 42% identical to the C-terminal half of the α subunit of CODH/ACS from the acetogenic anaerobe Clostridium thermoaceticum. Sequence analysis suggested potential structures and functions for the previously uncharacterized β subunit from M. thermophila. The deduced protein sequence of ORF1, located between the cdhC and cdhD genes, has 29% identity to NifH2 from Methanobacterium ivanovii.
AB - The cdhABC genes encoding the respective α, ε, and β subunits of the five-subunit (α, β, γ, δ, and ε) CO dehydrogenase/acetyl-coenzyme synthase (CODH/ACS) complex from Methanosarcina thermophila were cloned and sequenced. Northern (RNA) blot analyses indicated that the cdh genes encoding the five subunits and an open reading frame (ORF1) with unknown function are cotranscribed during growth on acetate. Northern blot and primer extension analyses suggested that mRNA processing and multiple promoters may be involved in cdh transcript synthesis. The putative CdhA (α subunit) and CdhB (ε subunit) proteins each have 40% identity to CdhA and CdhB of the CODH/ACS complex from Methanosaeta soehngenii. The cdhC gene encodes the subunit (CdhC) of the CODH/ACS complex from M. thermophila. The N-terminal 397 amino acids of CdhC are 42% identical to the C-terminal half of the α subunit of CODH/ACS from the acetogenic anaerobe Clostridium thermoaceticum. Sequence analysis suggested potential structures and functions for the previously uncharacterized β subunit from M. thermophila. The deduced protein sequence of ORF1, located between the cdhC and cdhD genes, has 29% identity to NifH2 from Methanobacterium ivanovii.
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U2 - 10.1128/jb.178.23.6849-6856.1996
DO - 10.1128/jb.178.23.6849-6856.1996
M3 - Article
C2 - 8955306
AN - SCOPUS:0029832099
SN - 0021-9193
VL - 178
SP - 6849
EP - 6856
JO - Journal of bacteriology
JF - Journal of bacteriology
IS - 23
ER -