Analysis of the isopentenyl disphosphate isomerase gene from Arabidopsis thaliana

Michael Campbell; Frederick M. Hahn; C. Dale Poulter; Thomas Leustek

doi:10.1023/A:1005935516274

Analysis of the isopentenyl disphosphate isomerase gene from Arabidopsis thaliana

Michael Campbell, Frederick M. Hahn, C. Dale Poulter, Thomas Leustek

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Abstract

Two Arabidopsis thaliana cDNAs (IPP1 and IPP2) encoding isopentenyl diphosphate isomerase (IPP isomerase) were isolated by complementation of an IPP isomerase mutant strain of Saccharomyces cerevisiae. Both cDNAs encode enzymes with an amino terminus that may function as a transit peptide for localization in plastids. At least 31 amino acids from the amino terminus of the IPP1 protein and 56 amino acids from the amino terminus of the IPP2 protein are not essential for enzymatic activity. Genomic DNA blot analysis confirmed that IPP1 and IPP2 are derived from a small gene family in A. thaliana. Based on northern analysis expression of both cDNAs occurs predominantly in roots of mature A. thaliana plants grown to the pre-flowering stage.

Original language	English (US)
Pages (from-to)	323-328
Number of pages	6
Journal	Plant molecular biology
Volume	36
Issue number	2
DOIs	https://doi.org/10.1023/A:1005935516274
State	Published - 1998

All Science Journal Classification (ASJC) codes

Agronomy and Crop Science
Genetics
Plant Science

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title = "Analysis of the isopentenyl disphosphate isomerase gene from Arabidopsis thaliana",

abstract = "Two Arabidopsis thaliana cDNAs (IPP1 and IPP2) encoding isopentenyl diphosphate isomerase (IPP isomerase) were isolated by complementation of an IPP isomerase mutant strain of Saccharomyces cerevisiae. Both cDNAs encode enzymes with an amino terminus that may function as a transit peptide for localization in plastids. At least 31 amino acids from the amino terminus of the IPP1 protein and 56 amino acids from the amino terminus of the IPP2 protein are not essential for enzymatic activity. Genomic DNA blot analysis confirmed that IPP1 and IPP2 are derived from a small gene family in A. thaliana. Based on northern analysis expression of both cDNAs occurs predominantly in roots of mature A. thaliana plants grown to the pre-flowering stage.",

author = "Michael Campbell and Hahn, {Frederick M.} and Poulter, {C. Dale} and Thomas Leustek",

note = "Funding Information: This project was supported in part by a grant from the National Institute of Health (GM 25521 CDP).",

year = "1998",

doi = "10.1023/A:1005935516274",

language = "English (US)",

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pages = "323--328",

journal = "Plant molecular biology",

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T1 - Analysis of the isopentenyl disphosphate isomerase gene from Arabidopsis thaliana

AU - Campbell, Michael

AU - Hahn, Frederick M.

AU - Poulter, C. Dale

AU - Leustek, Thomas

N1 - Funding Information: This project was supported in part by a grant from the National Institute of Health (GM 25521 CDP).

PY - 1998

Y1 - 1998

N2 - Two Arabidopsis thaliana cDNAs (IPP1 and IPP2) encoding isopentenyl diphosphate isomerase (IPP isomerase) were isolated by complementation of an IPP isomerase mutant strain of Saccharomyces cerevisiae. Both cDNAs encode enzymes with an amino terminus that may function as a transit peptide for localization in plastids. At least 31 amino acids from the amino terminus of the IPP1 protein and 56 amino acids from the amino terminus of the IPP2 protein are not essential for enzymatic activity. Genomic DNA blot analysis confirmed that IPP1 and IPP2 are derived from a small gene family in A. thaliana. Based on northern analysis expression of both cDNAs occurs predominantly in roots of mature A. thaliana plants grown to the pre-flowering stage.

AB - Two Arabidopsis thaliana cDNAs (IPP1 and IPP2) encoding isopentenyl diphosphate isomerase (IPP isomerase) were isolated by complementation of an IPP isomerase mutant strain of Saccharomyces cerevisiae. Both cDNAs encode enzymes with an amino terminus that may function as a transit peptide for localization in plastids. At least 31 amino acids from the amino terminus of the IPP1 protein and 56 amino acids from the amino terminus of the IPP2 protein are not essential for enzymatic activity. Genomic DNA blot analysis confirmed that IPP1 and IPP2 are derived from a small gene family in A. thaliana. Based on northern analysis expression of both cDNAs occurs predominantly in roots of mature A. thaliana plants grown to the pre-flowering stage.

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JO - Plant molecular biology

JF - Plant molecular biology

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Analysis of the isopentenyl disphosphate isomerase gene from Arabidopsis thaliana

Abstract

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