TY - JOUR
T1 - Analytical Py-GC/MS of Genetically Modified Poplar for the Increased Production of Bio-aromatics
AU - SriBala, Gorugantu
AU - Toraman, Hilal Ezgi
AU - Symoens, Steffen
AU - Déjardin, Annabelle
AU - Pilate, Gilles
AU - Boerjan, Wout
AU - Ronsse, Frederik
AU - Van Geem, Kevin M.
AU - Marin, Guy B.
N1 - Funding Information:
The authors thank Lennart Hoengenaert from VIB and Diana Vargas from LCT for their assistance in thioacidolysis experiments. The research leading to these results has received funding from the European Research Council under the European Union 's Seventh Framework Programme ( FP7/2007-2013 )/ ERC grant agreement n° 290793 and the “Long-Term Structural Methusalem Funding by the Flemish Government ”. The SBO proposal “Bioleum” supported by the Institute for Promotion of Innovation through Science and Technology in Flanders (IWT) is also acknowledged. The GBFOR experimental unit as well as Kevin Ader from Genobois platform (INRA Val de Loire) are warmly acknowledged for their involvement in the setting and maintenance of the GM poplar field trial, as well as the harvesting and pre-processing of the wood samples. This work has been funded by the FP7-KBBE-#211917 EnergyPoplar project.
Funding Information:
The authors thank Lennart Hoengenaert from VIB and Diana Vargas from LCT for their assistance in thioacidolysis experiments. The research leading to these results has received funding from the European Research Council under the European Union's Seventh Framework Programme (FP7/2007-2013)/ERC grant agreement n° 290793 and the “Long-Term Structural Methusalem Funding by the Flemish Government”. The SBO proposal “Bioleum” supported by the Institute for Promotion of Innovation through Science and Technology in Flanders (IWT) is also acknowledged. The GBFOR experimental unit as well as Kevin Ader from Genobois platform (INRA Val de Loire) are warmly acknowledged for their involvement in the setting and maintenance of the GM poplar field trial, as well as the harvesting and pre-processing of the wood samples. This work has been funded by the FP7-KBBE-#211917 EnergyPoplar project.
Publisher Copyright:
© 2019 The Authors
PY - 2019
Y1 - 2019
N2 - Genetic engineering is a powerful tool to steer bio-oil composition towards the production of speciality chemicals such as guaiacols, syringols, phenols, and vanillin through well-defined biomass feedstocks. Our previous work demonstrated the effects of lignin biosynthesis gene modification on the pyrolysis vapour compositions obtained from wood derived from greenhouse-grown poplars. In this study, field-grown poplars downregulated in the genes encoding CINNAMYL ALCOHOL DEHYDROGENASE (CAD), CAFFEIC ACID O-METHYLTRANSFERASE (COMT) and CAFFEOYL-CoA O-METHYLTRANSFERASE (CCoAOMT), and their corresponding wild type were pyrolysed in a Py-GC/MS. This work aims at capturing the effects of downregulation of the three enzymes on bio-oil composition using principal component analysis (PCA). 3,5-methoxytoluene, vanillin, coniferyl alcohol, 4-vinyl guaiacol, syringol, syringaldehyde, and guaiacol are the determining factors in the PCA analysis that are the substantially affected by COMT, CAD and CCoAOMT enzyme downregulation. COMT and CAD downregulated transgenic lines proved to be statistically different from the wild type because of a substantial difference in S and G lignin units. The sCAD line lead to a significant drop (nearly 51%) in S-lignin derived compounds, while CCoAOMT downregulation affected the least (7–11%). Further, removal of extractives via pretreatment enhanced the statistical differences among the CAD transgenic lines and its wild type. On the other hand, COMT downregulation caused 2-fold reduction in S-derived compounds compared to G-derived compounds. This study manifests the applicability of PCA analysis in tracking the biological changes in biomass (poplar in this case) and their effects on pyrolysis-oil compositions.
AB - Genetic engineering is a powerful tool to steer bio-oil composition towards the production of speciality chemicals such as guaiacols, syringols, phenols, and vanillin through well-defined biomass feedstocks. Our previous work demonstrated the effects of lignin biosynthesis gene modification on the pyrolysis vapour compositions obtained from wood derived from greenhouse-grown poplars. In this study, field-grown poplars downregulated in the genes encoding CINNAMYL ALCOHOL DEHYDROGENASE (CAD), CAFFEIC ACID O-METHYLTRANSFERASE (COMT) and CAFFEOYL-CoA O-METHYLTRANSFERASE (CCoAOMT), and their corresponding wild type were pyrolysed in a Py-GC/MS. This work aims at capturing the effects of downregulation of the three enzymes on bio-oil composition using principal component analysis (PCA). 3,5-methoxytoluene, vanillin, coniferyl alcohol, 4-vinyl guaiacol, syringol, syringaldehyde, and guaiacol are the determining factors in the PCA analysis that are the substantially affected by COMT, CAD and CCoAOMT enzyme downregulation. COMT and CAD downregulated transgenic lines proved to be statistically different from the wild type because of a substantial difference in S and G lignin units. The sCAD line lead to a significant drop (nearly 51%) in S-lignin derived compounds, while CCoAOMT downregulation affected the least (7–11%). Further, removal of extractives via pretreatment enhanced the statistical differences among the CAD transgenic lines and its wild type. On the other hand, COMT downregulation caused 2-fold reduction in S-derived compounds compared to G-derived compounds. This study manifests the applicability of PCA analysis in tracking the biological changes in biomass (poplar in this case) and their effects on pyrolysis-oil compositions.
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U2 - 10.1016/j.csbj.2019.04.007
DO - 10.1016/j.csbj.2019.04.007
M3 - Article
C2 - 31080566
AN - SCOPUS:85064979042
SN - 2001-0370
VL - 17
SP - 599
EP - 610
JO - Computational and Structural Biotechnology Journal
JF - Computational and Structural Biotechnology Journal
ER -