Antibacterial gene transfer across the tree of life

Jason A. Metcalf, Lisa J. Funkhouser-Jones, Kristen Brileya, Anna Louise Reysenbach, Seth R. Bordenstein

Research output: Contribution to journalArticlepeer-review

60 Scopus citations

Abstract

Though horizontal gene transfer (HGT) is widespread, genes and taxa experience biased rates of transferability. Curiously, independent transmission of homologous DNA to archaea, bacteria, eukaryotes, and viruses is extremely rare and often defies ecological and functional explanations. Here, we demonstrate that a bacterial lysozyme family integrated independently in all domains of life across diverse environments, generating the only glycosyl hydrolase 25 muramidases in plants and archaea. During coculture of a hydrothermal vent archaeon with a bacterial competitor, muramidase transcription is upregulated. Moreover, recombinant lysozyme exhibits broad-spectrum antibacterial action in a dose-dependent manner. Similar to bacterial transfer of antibiotic resistance genes, transfer of a potent antibacterial gene across the universal tree seemingly bestows a niche-transcending adaptation that trumps the barriers against parallel HGT to all domains. The discoveries also comprise the first characterization of an antibacterial gene in archaea and support the pursuit of antibiotics in this underexplored group.

Original languageEnglish (US)
Article numbere04266
Pages (from-to)1-18
Number of pages18
JournaleLife
Volume3
Issue numberNovember
DOIs
StatePublished - Nov 25 2014

All Science Journal Classification (ASJC) codes

  • General Neuroscience
  • General Immunology and Microbiology
  • General Biochemistry, Genetics and Molecular Biology

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