Antisera Recognising HLA‐A, ‐B and DRW Antigens Raised in Rhesus Monkeys

C. J. Barnstable, D. Snary, M. J. Crumpton, H. Balner, W. F. Bodmer

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Rhesus monkeys were immunized with partially purified HLA‐A, ‐B, ‐C and DR antigens. The resulting sera were shown to have activity against species‐specific determinants on both HLA‐A, ‐B, ‐C chains and β32 microglobulin by the use of somatic cell hybrids. When this was removed by absorption, the sera showed activity against three of the four HLA‐A and ‐B antigens in the immunogen when tested on a panel of peripheral blood lymphocytes and T cells. Antibodies recognizing HLA‐DR antigens were detected by testing platelet absorbed sera on a panel of typed lymphoblastoid cell lines. After absorption to remove activity against species‐specific determinants on the HLA‐DR antigens, two cross reacting specificities were defined. One consisted of a determinant in common between HLA‐DRwl, 2 and 6 and the other a putative determinant in common between HLA‐ DRw4, and 5. The nature and significance of these cross‐reacting groups of HLA‐DR antigens is discussed in the light of current HLA‐DR serology and the nature of HLA antigens in general.

Original languageEnglish (US)
Pages (from-to)407-418
Number of pages12
JournalTissue Antigens
Issue number5
StatePublished - May 1980

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Genetics


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