Abstract
An acousto-optic tunable filter (AOTF) has been used to perform multicolor fluorescence detection for four and five-color short tandem repeat (STR) analysis on glass microchips. Matrix files were initially generated by collecting and comparing the laser-induced fluorescence emission of the labels specific to a particular STR kit, and raw data was processed to remove spectral overlap. The AmpFlSTR® kits used in this work include Profiler Plus™ and COfiler™, which are four-color kits used in tandem to address the core STR loci, as well as the five-color Identifiler™ kit, which contains each of the loci. In contrast to previous reports on multicolor detection for STR analysis on microchips, this detection system is characterized by a single filter and detector, and reports the first five-color genotyping application on-chip. This capability matches the portability and reduced scale of the microchip with the state-of-the-art in multicolor STR analysis kits.
Original language | English (US) |
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Pages (from-to) | 1285-1291 |
Number of pages | 7 |
Journal | Lab on a Chip |
Volume | 8 |
Issue number | 8 |
DOIs | |
State | Published - 2008 |
All Science Journal Classification (ASJC) codes
- Bioengineering
- Biochemistry
- General Chemistry
- Biomedical Engineering