TY - JOUR
T1 - Application of bioaffinity mass spectrometry for analysis of ligands
AU - Zhu, Yusheng
AU - Valdes, Roland
AU - Jortani, Saeed A.
PY - 2005/12/1
Y1 - 2005/12/1
N2 - Bioaffinity mass spectrometry is a novel technology for analysis of binding proteins and their ligands. In this review, we introduce the concepts and principles of bioaffinity surface-enhanced laser desorption/ionization-time of flight mass spectrometry (SELDI-TOF MS). Various preactivated chip types and several approaches for binding of ligands or their binders to the chips are discussed. We also provide specific examples for the use of this technology for screening antibodies, analyzing ligands, glycoconjugates, protein-protein interactions, and DNA (RNA) binding proteins. In pursuit of developing new tests or studies of mechanism of drug action in therapeutic drug monitoring practice, this technology may provide a more rapid approach for ligand-binder studies.
AB - Bioaffinity mass spectrometry is a novel technology for analysis of binding proteins and their ligands. In this review, we introduce the concepts and principles of bioaffinity surface-enhanced laser desorption/ionization-time of flight mass spectrometry (SELDI-TOF MS). Various preactivated chip types and several approaches for binding of ligands or their binders to the chips are discussed. We also provide specific examples for the use of this technology for screening antibodies, analyzing ligands, glycoconjugates, protein-protein interactions, and DNA (RNA) binding proteins. In pursuit of developing new tests or studies of mechanism of drug action in therapeutic drug monitoring practice, this technology may provide a more rapid approach for ligand-binder studies.
UR - http://www.scopus.com/inward/record.url?scp=33646832338&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33646832338&partnerID=8YFLogxK
U2 - 10.1097/01.ftd.0000179851.32093.dc
DO - 10.1097/01.ftd.0000179851.32093.dc
M3 - Article
C2 - 16404796
AN - SCOPUS:33646832338
SN - 0163-4356
VL - 27
SP - 694
EP - 699
JO - Therapeutic Drug Monitoring
JF - Therapeutic Drug Monitoring
IS - 6
ER -