TY - JOUR
T1 - Application of periodic backpulsing to reduce membrane fouling during ultrafiltration of plasmid DNA
AU - Borujeni, Ehsan Espah
AU - Li, Ying
AU - Zydney, Andrew L.
N1 - Funding Information:
This work was supported in part by Grant kbp plasmid and David Currie for his help in conducting some of the experiments and samples analysis. CBET 0755816 from the National Science Foundation . The authors acknowledge Jeffrey Chamberlain from the University of Washington who provided the 9.8
Publisher Copyright:
© 2014 Elsevier B.V.
PY - 2015/1/1
Y1 - 2015/1/1
N2 - There is considerable interest in using ultrafiltration for the purification of plasmid DNA, but membrane fouling remains an issue. The objective of this work was to examine the use of periodic backpulsing to reduce fouling during plasmid ultrafiltration by "releasing" previously trapped plasmids from the membrane pores. Experiments were performed using hollow fiber ultrafiltration membranes over a range of backpulse frequency, amplitude, and duration. Plasmid removal under typical conditions was dominated by the shear / drag associated with the tangential flow, with good recovery of plasmid transmission obtained by periodically closing the permeate exit line (without any backpressure). Data were analyzed using a simple fouling model that accounts for the partial blockage of pores by trapped plasmids that are then released during backpulsing. The results clearly demonstrate that this type of pulsed operation can provide much greater plasmid recovery in the permeate solution with a nearly constant filtrate flux during a diafiltration process.
AB - There is considerable interest in using ultrafiltration for the purification of plasmid DNA, but membrane fouling remains an issue. The objective of this work was to examine the use of periodic backpulsing to reduce fouling during plasmid ultrafiltration by "releasing" previously trapped plasmids from the membrane pores. Experiments were performed using hollow fiber ultrafiltration membranes over a range of backpulse frequency, amplitude, and duration. Plasmid removal under typical conditions was dominated by the shear / drag associated with the tangential flow, with good recovery of plasmid transmission obtained by periodically closing the permeate exit line (without any backpressure). Data were analyzed using a simple fouling model that accounts for the partial blockage of pores by trapped plasmids that are then released during backpulsing. The results clearly demonstrate that this type of pulsed operation can provide much greater plasmid recovery in the permeate solution with a nearly constant filtrate flux during a diafiltration process.
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U2 - 10.1016/j.memsci.2014.08.059
DO - 10.1016/j.memsci.2014.08.059
M3 - Article
AN - SCOPUS:84907745065
SN - 0376-7388
VL - 473
SP - 102
EP - 108
JO - Journal of Membrane Science
JF - Journal of Membrane Science
ER -