Application of quantitative RT-PCR to the analysis of dopamine receptor mRNA levels in rat striatum

Keyphrases

• Quantitative PCR 100%
• MRNA Level 100%
• Dopamine Receptor 100%
• Rat Striatum 100%
• RNA Preparation 50%
• Digestion 25%
• Direct Analysis 25%
• Adaptation 25%
• Signal-to-noise Ratio 25%
• Reaction Conditions 25%
• Annealing 25%
• Point mutation 25%
• CDNA Clone 25%
• Dopamine D2 Receptor (DRD2) 25%
• Melting Temperature 25%
• D2 Dopamine Receptor 25%
• Primer Pairs 25%
• Brain Tissue 25%
• Restriction Sites 25%
• Radioactivity 25%
• Linear Response 25%
• Restriction Enzyme 25%
• PCR Cycles 25%
• Transcribed RNA 25%
• Contaminant Effect 25%

Biochemistry, Genetics and Molecular Biology

• RNA 100%
• Dopamine Receptor 100%
• Quantitative Reverse Transcription Polymerase Chain Reaction 100%
• Dopamine Receptor D2 40%
• Reverse Transcription Polymerase Chain Reaction 40%
• Polyacrylamide Gel Electrophoresis 20%
• Isoform 20%
• Point Mutation 20%
• Molecular Cloning 20%
• Melting Temperature 20%
• Restriction Site 20%
• Signal Noise Ratio 20%
• Enzyme Isolation 20%
• Radioactivity 20%
• Restriction Enzyme 20%

Neuroscience

• Dopamine Receptor 100%
• Striatum 100%
• Reverse Transcription Polymerase Chain Reaction 100%
• Dopamine Receptor D2 50%
• In Vitro 25%
• Complementary DNA 25%
• Receptor Subtype 25%
• Polyacrylamide Gel Electrophoresis 25%
• Restriction Endonuclease 25%
• Point Mutation 25%
• Signal-to-Noise Ratio 25%