Application of RAPDs to DNA extracted from apple rootstocks

Wesley R. Autio; James R. Schupp; David C. Ferree; Richard Glavin; David L. Mulcahy

Application of RAPDs to DNA extracted from apple rootstocks

Wesley R. Autio, James R. Schupp, David C. Ferree, Richard Glavin, David L. Mulcahy

Plant Science

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Samples of 15 different rootstocks from mature apple trees (Malus xdomestica Borkh.) with 'Starkspur Supreme Delicious' as the scion were differentiated adequately using random amplified polymorphic DNAs (RAPDs). A procedure for extraction of DNA from root material is described. Patterns of DNA from leaf tissue of young trees of 10 of the rootstocks were compared with those from root tissue of mature trees grafted on the same rootstocks and at a different location to reaffirm identification of these rootstocks. Similar patterns were obtained for a) root vs. leaf tissue and b) tissue from two locations. Except for one apparent misidentification (within a single replication), patterns for root tissue of all rootstocks matched those of the corresponding leaf tissue.

Original language	English (US)
Pages (from-to)	333-335
Number of pages	3
Journal	HortScience
Volume	33
Issue number	2
State	Published - 1998

All Science Journal Classification (ASJC) codes

Horticulture

Cite this

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title = "Application of RAPDs to DNA extracted from apple rootstocks",

abstract = "Samples of 15 different rootstocks from mature apple trees (Malus xdomestica Borkh.) with 'Starkspur Supreme Delicious' as the scion were differentiated adequately using random amplified polymorphic DNAs (RAPDs). A procedure for extraction of DNA from root material is described. Patterns of DNA from leaf tissue of young trees of 10 of the rootstocks were compared with those from root tissue of mature trees grafted on the same rootstocks and at a different location to reaffirm identification of these rootstocks. Similar patterns were obtained for a) root vs. leaf tissue and b) tissue from two locations. Except for one apparent misidentification (within a single replication), patterns for root tissue of all rootstocks matched those of the corresponding leaf tissue.",

author = "Autio, {Wesley R.} and Schupp, {James R.} and Ferree, {David C.} and Richard Glavin and Mulcahy, {David L.}",

year = "1998",

language = "English (US)",

volume = "33",

pages = "333--335",

journal = "HortScience",

issn = "0018-5345",

publisher = "American Society for Horticultural Science",

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TY - JOUR

T1 - Application of RAPDs to DNA extracted from apple rootstocks

AU - Autio, Wesley R.

AU - Schupp, James R.

AU - Ferree, David C.

AU - Glavin, Richard

AU - Mulcahy, David L.

PY - 1998

Y1 - 1998

N2 - Samples of 15 different rootstocks from mature apple trees (Malus xdomestica Borkh.) with 'Starkspur Supreme Delicious' as the scion were differentiated adequately using random amplified polymorphic DNAs (RAPDs). A procedure for extraction of DNA from root material is described. Patterns of DNA from leaf tissue of young trees of 10 of the rootstocks were compared with those from root tissue of mature trees grafted on the same rootstocks and at a different location to reaffirm identification of these rootstocks. Similar patterns were obtained for a) root vs. leaf tissue and b) tissue from two locations. Except for one apparent misidentification (within a single replication), patterns for root tissue of all rootstocks matched those of the corresponding leaf tissue.

AB - Samples of 15 different rootstocks from mature apple trees (Malus xdomestica Borkh.) with 'Starkspur Supreme Delicious' as the scion were differentiated adequately using random amplified polymorphic DNAs (RAPDs). A procedure for extraction of DNA from root material is described. Patterns of DNA from leaf tissue of young trees of 10 of the rootstocks were compared with those from root tissue of mature trees grafted on the same rootstocks and at a different location to reaffirm identification of these rootstocks. Similar patterns were obtained for a) root vs. leaf tissue and b) tissue from two locations. Except for one apparent misidentification (within a single replication), patterns for root tissue of all rootstocks matched those of the corresponding leaf tissue.

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M3 - Article

AN - SCOPUS:0031980978

SN - 0018-5345

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JO - HortScience

JF - HortScience

IS - 2

ER -

Application of RAPDs to DNA extracted from apple rootstocks

Abstract

All Science Journal Classification (ASJC) codes

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