TY - GEN
T1 - Aptamer-based nanofluidics for the molecular detection in ultra-small volume
AU - Yang, Jinbin
AU - Kamai, Hiroki
AU - Wang, Yong
AU - Xu, Yan
N1 - Publisher Copyright:
© 2020 CBMS-0001
PY - 2020
Y1 - 2020
N2 - Molecular detection in samples with sub-picoliter (picoliter, pL =10−12 L) volumes is a challenge impeding the development of several fields such as single cell studies at the single molecule level. As a proof-of-concept study, here we proposed the aptamer-based nanofluidic device for the detection in samples with femtoliter (fL, 10−15 L) volumes. Platelet-derived growth factor BB (PDGF-BB) and its aptamers were used. The aptamers were modified onto the gold nanopattern surfaces inside nanochannels, and could recognize targets with high selectivity. This study proved that the aptamer-based nanofluidics is promising in bioanalysis area especially in the single-cell omics studies.
AB - Molecular detection in samples with sub-picoliter (picoliter, pL =10−12 L) volumes is a challenge impeding the development of several fields such as single cell studies at the single molecule level. As a proof-of-concept study, here we proposed the aptamer-based nanofluidic device for the detection in samples with femtoliter (fL, 10−15 L) volumes. Platelet-derived growth factor BB (PDGF-BB) and its aptamers were used. The aptamers were modified onto the gold nanopattern surfaces inside nanochannels, and could recognize targets with high selectivity. This study proved that the aptamer-based nanofluidics is promising in bioanalysis area especially in the single-cell omics studies.
UR - http://www.scopus.com/inward/record.url?scp=85098285588&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85098285588&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85098285588
T3 - MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 354
EP - 355
BT - MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
Y2 - 4 October 2020 through 9 October 2020
ER -