TY - JOUR
T1 - Arabidopsis interdigitating cell growth requires two antagonistic pathways with opposing action on cell morphogenesis
AU - Fu, Ying
AU - Gu, Ying
AU - Zheng, Zhiliang
AU - Wasteneys, Geoffrey
AU - Yang, Zhenbiao
N1 - Funding Information:
We thank Bo Liu, Sidney Shaw, and Andre Jagendorf for their critical comments on the manuscript; the Salk Institute Genomic Analysis Laboratory for providing the sequence-indexed Arabidopsis T-DNA insertion mutants; John Fowler for providing pBS-CFP vector; and Albrecht Arnim for pBS35S:YFP vector. This work is supported by NSF grant (IBN-0417255) to Z.Y.
PY - 2005/3/11
Y1 - 2005/3/11
N2 - Coordinating growth and communication between adjacent cells is a critical yet poorly understood aspect of tissue development and organ morphogenesis. We report a Rho GTPase signaling network underlying the jigsaw puzzle appearance of Arabidopsis leaf pavement cells, in which localized outgrowth in one cell is coordinated with localized inhibition of outgrowth of the adjacent cell to form interdigitating lobes and indentations. Locally activated ROP2, a Rho-related GTPase from plants, activates RIC4 to promote the assembly of cortical actin microfilaments required for localized outgrowth. Meanwhile, ROP2 inactivates another target RIC1, whose activity promotes well-ordered cortical microtubules. RIC1-dependent microtubule organization not only locally inhibits outgrowth but in turn suppresses ROP2 activation in the indentation zones. Thus, outgrowth-promoting ROP2 and outgrowth-inhibiting RIC1 pathways antagonize each other. We propose that the counteractivity of these two pathways demarcates outgrowing and indenting cortical domains, coordinating a process that gives rise to interdigitations between adjacent pavement cells.
AB - Coordinating growth and communication between adjacent cells is a critical yet poorly understood aspect of tissue development and organ morphogenesis. We report a Rho GTPase signaling network underlying the jigsaw puzzle appearance of Arabidopsis leaf pavement cells, in which localized outgrowth in one cell is coordinated with localized inhibition of outgrowth of the adjacent cell to form interdigitating lobes and indentations. Locally activated ROP2, a Rho-related GTPase from plants, activates RIC4 to promote the assembly of cortical actin microfilaments required for localized outgrowth. Meanwhile, ROP2 inactivates another target RIC1, whose activity promotes well-ordered cortical microtubules. RIC1-dependent microtubule organization not only locally inhibits outgrowth but in turn suppresses ROP2 activation in the indentation zones. Thus, outgrowth-promoting ROP2 and outgrowth-inhibiting RIC1 pathways antagonize each other. We propose that the counteractivity of these two pathways demarcates outgrowing and indenting cortical domains, coordinating a process that gives rise to interdigitations between adjacent pavement cells.
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U2 - 10.1016/j.cell.2004.12.026
DO - 10.1016/j.cell.2004.12.026
M3 - Article
C2 - 15766531
AN - SCOPUS:14844304308
SN - 0092-8674
VL - 120
SP - 687
EP - 700
JO - Cell
JF - Cell
IS - 5
ER -