@article{295a940e2ab046d0ae9a9f0736984190,
title = "Architecture and dynamics of a desmosome–endoplasmic reticulum complex",
abstract = "The endoplasmic reticulum (ER) forms a dynamic network that contacts other cellular membranes to regulate stress responses, calcium signalling and lipid transfer. Here, using high-resolution volume electron microscopy, we find that the ER forms a previously unknown association with keratin intermediate filaments and desmosomal cell–cell junctions. Peripheral ER assembles into mirror image-like arrangements at desmosomes and exhibits nanometre proximity to keratin filaments and the desmosome cytoplasmic plaque. ER tubules exhibit stable associations with desmosomes, and perturbation of desmosomes or keratin filaments alters ER organization, mobility and expression of ER stress transcripts. These findings indicate that desmosomes and the keratin cytoskeleton regulate the distribution, function and dynamics of the ER network. Overall, this study reveals a previously unknown subcellular architecture defined by the structural integration of ER tubules with an epithelial intercellular junction.",
author = "Bharathan, {Navaneetha Krishnan} and William Giang and Hoffman, {Coryn L.} and Aaron, {Jesse S.} and Satya Khuon and Chew, {Teng Leong} and {COSEM Project Team} and Stephan Preibisch and Trautman, {Eric T.} and Larissa Heinrich and John Bogovic and Davis Bennett and David Ackerman and Woohyun Park and Alyson Petruncio and Weigel, {Aubrey V.} and Stephan Saalfeld and {Wayne Vogl}, A. and Stahley, {Sara N.} and Kowalczyk, {Andrew P.}",
note = "Funding Information: The authors are grateful to D. Lerit (Emory University), K. Green and L. Godsel (Northwestern University), T. Magin (University of Leipzig), M. Amagai (Keio University, Tokyo) and A. Nelson and R. Hobbs (Penn State College of Medicine) for instrument use, reagents and advice. We thank N. Sheaffer and J. Bednarczyk from Penn State College of Medicine{\textquoteright}s Flow Cytometry Core for assistance with cell sorting. This research project was supported in part by the Emory University Integrated Cellular Imaging Core. This work was supported by: National Institutes of Health grant R01AR048266 (A.P.K.); Natural Sciences and Engineering Research Council of Canada Discovery Grant RGPIN-2018-14 03727 (A.W.V.). Cryo-SIM and FIB-SEM imaging were done in collaboration with the Advanced Imaging Center at Janelia Research Campus, a facility jointly supported by the Gordon and Betty Moore Foundation and the Howard Hughes Medical Institute. Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive licence to Springer Nature Limited.",
year = "2023",
month = jun,
doi = "10.1038/s41556-023-01154-4",
language = "English (US)",
volume = "25",
pages = "823--835",
journal = "Nature Cell Biology",
issn = "1465-7392",
publisher = "Nature Research",
number = "6",
}