TY - JOUR
T1 - Arthropod-bacteria interactions influence assembly of aquatic host microbiome and pathogen defense
AU - Greenspan, Sasha E.
AU - Lyra, Mariana L.
AU - Migliorini, Gustavo H.
AU - Kersch-Becker, Mônica F.
AU - Bletz, Molly C.
AU - Lisboa, Cybele Sabino
AU - Pontes, Mariana R.
AU - Ribeiro, Luisa P.
AU - Neely, Wesley J.
AU - Rezende, Felipe
AU - Romero, Gustavo Q.
AU - Woodhams, Douglas C.
AU - Haddad, Célio F.B.
AU - Toledo, Luís Felipe
AU - Guilherme Becker, C.
N1 - Funding Information:
São Paulo Research Foundation (FAPESP) provided grants to L.F.T. (no. 2014/23388-7) and C.F.B.H. (no. 2013/50741-7 and no. 2014/50342-8) and research fellowships to M.L.L. (no. 2017/26162-8), L.P.R. (no. 2016/03344-0), and M.R.P. (CAPES no. 001). National Council of Technological and Scientific Development (CNPq) provided research fellowships to C.G.B. (no. 312895/2014-3), L.F.T. (no. 300896/2016-6) and C.F.B.H. (no. 302518/2013-4).
Publisher Copyright:
© 2019 The Author(s) Published by the Royal Society. All rights reserved.
PY - 2019/6/26
Y1 - 2019/6/26
N2 - The host-associated microbiome is vital to host immunity and pathogen defense. In aquatic ecosystems, organisms may interact with environmental bacteria to influence the pool of potential symbionts, but the effects of these interactions on host microbiome assembly and pathogen resistance are unresolved. We used replicated bromeliad microecosystems to test for indirect effects of arthropod - bacteria interactions on host microbiome assembly and pathogen burden, using tadpoles and the fungal amphibian pathogen Batrachochytrium dendrobatidis as a model host - pathogen system. Arthropods influenced host microbiome assembly by altering the pool of environmental bacteria, with arthropod - bacteria interactions specifically reducing host colonization by transient bacteria and promoting antimicrobial components of aquatic bacterial communities. Arthropods also reduced fungal zoospores in the environment, but fungal infection burdens in tadpoles corresponded most closely with arthropod-mediated patterns in microbiome assembly. This result indicates that the cascading effects of arthropods on the maintenance of a protective host microbiome may be more strongly linked to host health than negative effects of arthropods on pools of pathogenic zoospores. Our work reveals tight links between healthy ecosystem dynamics and the functioning of host microbiomes, suggesting that ecosystem disturbances such as loss of arthropods may have downstream effects on host-associated microbial pathogen defenses and host fitness.
AB - The host-associated microbiome is vital to host immunity and pathogen defense. In aquatic ecosystems, organisms may interact with environmental bacteria to influence the pool of potential symbionts, but the effects of these interactions on host microbiome assembly and pathogen resistance are unresolved. We used replicated bromeliad microecosystems to test for indirect effects of arthropod - bacteria interactions on host microbiome assembly and pathogen burden, using tadpoles and the fungal amphibian pathogen Batrachochytrium dendrobatidis as a model host - pathogen system. Arthropods influenced host microbiome assembly by altering the pool of environmental bacteria, with arthropod - bacteria interactions specifically reducing host colonization by transient bacteria and promoting antimicrobial components of aquatic bacterial communities. Arthropods also reduced fungal zoospores in the environment, but fungal infection burdens in tadpoles corresponded most closely with arthropod-mediated patterns in microbiome assembly. This result indicates that the cascading effects of arthropods on the maintenance of a protective host microbiome may be more strongly linked to host health than negative effects of arthropods on pools of pathogenic zoospores. Our work reveals tight links between healthy ecosystem dynamics and the functioning of host microbiomes, suggesting that ecosystem disturbances such as loss of arthropods may have downstream effects on host-associated microbial pathogen defenses and host fitness.
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U2 - 10.1098/rspb.2019.0924
DO - 10.1098/rspb.2019.0924
M3 - Article
C2 - 31238845
AN - SCOPUS:85068414967
SN - 0962-8452
VL - 286
JO - Proceedings of the Royal Society B: Biological Sciences
JF - Proceedings of the Royal Society B: Biological Sciences
IS - 1905
M1 - 20190924
ER -