TY - JOUR
T1 - Aspartic proteinase genes in the brassicaceae Arabidopsis thaliana and Brassica napus
AU - D'Hondt, Kathleen
AU - Stack, Sylvia
AU - Gutteridge, Steve
AU - Vandekerckhove, Joel
AU - Krebbers, Enno
AU - Gal, Susannah
N1 - Funding Information:
We thank Jef Seurinck for sequencing pKD420 and pKD421, Rikus Pomp for help in the early stages of sequencing pKD425, and Jose Van Damme for peptide sequencing. We would also like to thank Sandeep Gulati for help in isolating pSG2J2 and Jane Tien for help with some of the sequencing of this plasmid. S. Gal was supported in part by a Binghamton University Faculty Summer Research Support grant, while K.D. was supported by a grant from the Belgian IWT.
PY - 1997/1
Y1 - 1997/1
N2 - Active aspartic proteinase is isolated from Brassica napus seeds and the peptide sequence is used to generate primers for PCR. We present here cDNA and genomic clones for aspartic proteinases from the closely related Brassicaceae Arabidopsis thaliana and Brassica napus. The Arabidopsis cDNA represents a single gene, while Brassica has at least 4 genes. Like other plant aspartic proteases, the two Brassicaceae enzymes contain an extra protein domain of about 100 amino acids relative to the mammalian forms. The intron/exon arrangement in the Brassica genomic clone is significantly different from that in mammalian genes. As the proteinase is isolated from seeds, the same tissue where 2S albumins are processed, this implies expression of one of the aspartic proteinase genes there.
AB - Active aspartic proteinase is isolated from Brassica napus seeds and the peptide sequence is used to generate primers for PCR. We present here cDNA and genomic clones for aspartic proteinases from the closely related Brassicaceae Arabidopsis thaliana and Brassica napus. The Arabidopsis cDNA represents a single gene, while Brassica has at least 4 genes. Like other plant aspartic proteases, the two Brassicaceae enzymes contain an extra protein domain of about 100 amino acids relative to the mammalian forms. The intron/exon arrangement in the Brassica genomic clone is significantly different from that in mammalian genes. As the proteinase is isolated from seeds, the same tissue where 2S albumins are processed, this implies expression of one of the aspartic proteinase genes there.
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U2 - 10.1023/A:1005794917200
DO - 10.1023/A:1005794917200
M3 - Article
C2 - 9037171
AN - SCOPUS:0031040431
SN - 0167-4412
VL - 33
SP - 187
EP - 192
JO - Plant molecular biology
JF - Plant molecular biology
IS - 1
ER -